Saltar al contenido
Merck

HWGCRISPR

Sigma-Aldrich

Sigma Whole Human Genome Lentiviral CRISPR Pool

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

Código UNSPSC:
41106609
NACRES:
NA.51

envase

pkg of 8x25 μL (vials)

Nivel de calidad

concentración

5x108  VP/ml (via p24 assay)

aplicaciones

CRISPR

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

CRISPR (clustered regularly interspaced short palindromic repeat) are repeated sequence of DNA. These are transcribed in a single transcript and later divided into short repeats.

Aplicación

Functional Genomics/Screening /Target Validation

Acciones bioquímicas o fisiológicas

Utilize the power of CRISPR Whole Genome Lentiviral Pools to knockout and screen every gene in the human genome. The Sigma Whole Human Genome Lentiviral CRISPR Pool is provided as 200 ul in 8 x 25 ul aliquots at a minimum titer of 5x108 viral particles/ml via p24.

Características y beneficios

  • Use CRISPR nucleases to knockout protein-coding genes to assess their function
  • Efficiently screen the whole human genome (16,000+ genes) at the bench-top without robotics or specialized equipment
  • Increased flexibility consisting of 8 subpools at 23,000 clones per pool (184,000 clones total), from the previous 2 subpools (Gecko v2)
  • Expanded gRNA coverage at 10 gRNAs per gene, from the previous 6 gRNAs per gene (Gecko v2)
  • Numerous built-in enrichment and depletion controls allows researchers to confidently gauge the success of their pooled screening experiments
  • 2 Vector System (pools are gRNA-only, Cas9 sold separately)
  • Ease of optimization: Utilizes same vector system as Gecko v2 allowing for optimization one for both systems
  • Minimize off-targeting: stringent gRNA design and tiling rules

Nota de preparación

Puro Kill Curve and Determining CFU (Colony Formation Unit) per mL.
Prior to performing a library-scale screening, two preliminary experiments must be conducted: (1) determine the sensitivity of target cell type to puromycin (kill curve), and (2) determine the functional titer of the lentivirus in your cell type by completing a colony-forming assay (measured in CFU/ml). The calculation of MOI (multiplicity of infection) should be based on the value of CFU. Different cell types vary in transduction efficiency and different lentiviral constructs do not behave identically, so it is critical to optimize your experimental conditions with control lentiviral CRISPR clones (available from Sigma) prior to performing your pooled experiment.

Otras notas

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Campylobacter Ecology and Evolution (2014)

Artículos

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Ver todo

Protocolos

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Ver todo

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico