HWGCRISPR
Sigma Whole Human Genome Lentiviral CRISPR Pool
About This Item
Productos recomendados
envase
pkg of 8x25 μL (vials)
Nivel de calidad
concentración
5x108 VP/ml (via p24 assay)
aplicaciones
CRISPR
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Descripción general
Aplicación
Acciones bioquímicas o fisiológicas
Características y beneficios
- Use CRISPR nucleases to knockout protein-coding genes to assess their function
- Efficiently screen the whole human genome (16,000+ genes) at the bench-top without robotics or specialized equipment
- Increased flexibility consisting of 8 subpools at 23,000 clones per pool (184,000 clones total), from the previous 2 subpools (Gecko v2)
- Expanded gRNA coverage at 10 gRNAs per gene, from the previous 6 gRNAs per gene (Gecko v2)
- Numerous built-in enrichment and depletion controls allows researchers to confidently gauge the success of their pooled screening experiments
- 2 Vector System (pools are gRNA-only, Cas9 sold separately)
- Ease of optimization: Utilizes same vector system as Gecko v2 allowing for optimization one for both systems
- Minimize off-targeting: stringent gRNA design and tiling rules
Nota de preparación
Prior to performing a library-scale screening, two preliminary experiments must be conducted: (1) determine the sensitivity of target cell type to puromycin (kill curve), and (2) determine the functional titer of the lentivirus in your cell type by completing a colony-forming assay (measured in CFU/ml). The calculation of MOI (multiplicity of infection) should be based on the value of CFU. Different cell types vary in transduction efficiency and different lentiviral constructs do not behave identically, so it is critical to optimize your experimental conditions with control lentiviral CRISPR clones (available from Sigma) prior to performing your pooled experiment.
Otras notas
Información legal
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Protocolos
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
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