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S4019

Sigma-Aldrich

Bicarbonato de sodio

Hybri-Max™, powder, suitable for hybridoma, ≥99.5%

Sinónimos:

Sodium hydrogen carbonate

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About This Item

Fórmula lineal:
NaHCO3
Número de CAS:
144-55-8
Peso molecular:
84.01
Beilstein/REAXYS Number:
4153970
EC Number:
205-633-8
MDL number:
MFCD00003528
UNSPSC Code:
12161702
PubChem Substance ID:
57654616
NACRES:
NA.75

grade

Hybri-Max™

assay

≥99.5%

form

powder

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

pH

8.3 (0.1 molfreshly prepared)

pKa (25 °C)

(1) 6.37, (2) 10.25 (carbonic acid)

solubility

water: soluble 100 mg/mL

density

2.16 g/mL at 25 °C (lit.)

SMILES string

[Na+].OC([O-])=O

InChI

1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1

InChI key

UIIMBOGNXHQVGW-UHFFFAOYSA-M

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Application

Sodium bicarbonate has been used as a supplement:
  • in minimum essential medium (MEM)/flushing medium for embryo isolation
  • in TCM 199 medium for the collection of cumulus-oocyte complexes (COCs) and in vitro maturation (IVM)
  • in IVM medium for in vitro maturation of COCs

Biochem/physiol Actions

Sodium bicarbonate occurs naturally as the mineral nahcolite. It is used to prepare many sodium salts as well as a carbon dioxide source. Sodium bicarbonate is used in the manufacture of fire extinguishers and cleaning agents. Bicarbonate plays an important role in pH regulation in vivo. In biochemistry, molecular biology, and chromatography, sodium bicarbonate is used as a buffer. This Hybri-Max™ product is suitable for use in hybridoma and cell culture research (2.2 mg/ml).

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes
Development, 1, 5-14 (2010)
The effect of estrous ewe serum and heparin on in vitro fertilization and subsequent embryonic development in sheep
Li F, et al.
Small Ruminant Research, 63(3), 226-232 (2006)
Shay P McGuinness et al.
Critical care medicine, 41(7), 1599-1607 (2013-05-21)
Cardiac surgery-associated acute kidney injury occurs in up to 50% of patients and is associated with increased mortality and morbidity. This study aimed to discover if perioperative urinary alkalinization with sodium bicarbonate infusion reduces the prevalence of cardiac surgery-associated acute
Joshua C Tremblay et al.
The Journal of physiology, 593(3), 723-737 (2014-11-25)
Blood flow through intrapulmonary arteriovenous anastomoses (IPAVA) is increased by acute hypoxia during rest by unknown mechanisms. Oral administration of acetazolamide blunts the pulmonary vascular pressure response to acute hypoxia, thus permitting the observation of IPAVA blood flow with minimal
Sophia Zoungas et al.
Annals of internal medicine, 151(9), 631-638 (2009-11-04)
Intravenous sodium bicarbonate has been proposed to reduce the risk for contrast-induced nephropathy (CIN). To determine the effect of sodium bicarbonate on the risk for CIN. MEDLINE, PubMed, EMBASE, and the Cochrane Central Register of Controlled Trials from 1950 to
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