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S0146

Sigma-Aldrich

Schneider′s Insect Medium

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Sinónimos:

Schneider’s Drosophila Medium

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About This Item

MDL number:
MFCD00242089
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Schneider′s Insect Medium, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for insect cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | insect: suitable

suitability

suitable for culture of other dipteran cell lines (Originally developed for the culture of Drosophila cells)

components

NaHCO3: 0.4 g/L
glucose: 2 g/L (Dextro)
L-glutamine: 1.8 g/L

shipped in

ambient

storage temp.

2-8°C

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Application

Schneider′s insect medium has been used:
  • to culture Drosophila neuronal cell line (BG3-c2 cells)
  • to cultivate subcultures of Leishmania major for promastigote growth inhibition assay
  • to seed purified ookinetes with Drosophila melanogaster S2 cells to assess the effect of the avermectin compound on cell development and to promote oocyte development

Originally developed for the culture of Drosophila; suitable for culture of other dipteran cell lines.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Amit Sinha et al.
Genome biology and evolution, 11(3), 706-720 (2019-02-05)
Wolbachia, an alpha-proteobacterium closely related to Rickettsia, is a maternally transmitted, intracellular symbiont of arthropods and nematodes. Aedes albopictus mosquitoes are naturally infected with Wolbachia strains wAlbA and wAlbB. Cell line Aa23 established from Ae. albopictus embryos retains only wAlbB
Poonam Aggarwal et al.
Genetics, 207(1), 197-213 (2017-07-12)
One of the pertinent issues associated with cellular plasticity is to understand how the delicate balance between the determined state of cells and the extent to which they can transdetermine is maintained. Employing the well-established model of generating ectopic eyes
Amanda R Brock et al.
Genetics, 206(3), 1505-1520 (2017-05-18)
Regeneration is a complex process that requires an organism to recognize and repair tissue damage, as well as grow and pattern new tissue. Here, we describe a genetic screen to identify novel regulators of regeneration. We ablated the
Jeffrey S Dason et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(38), 23286-23291 (2019-06-20)
Painful or threatening experiences trigger escape responses that are guided by nociceptive neuronal circuitry. Although some components of this circuitry are known and conserved across animals, how this circuitry is regulated at the genetic and developmental levels is mostly unknown.
Inês M A Ribeiro et al.
Cell, 174(3), 607-621 (2018-07-24)
Many animals rely on vision to detect, locate, and track moving objects. In Drosophila courtship, males primarily use visual cues to orient toward and follow females and to select the ipsilateral wing for courtship song. Here, we show that the
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