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PKH26PCL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

Distributed for Phanos Technologies

Sinónimos:

Phagocytic cell label

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.32

Aplicación

This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.

Principio

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Ligadura / enlace

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Información legal

CellVue is a registered trademark of Phanos Technologies

Solo componentes del kit

Referencia del producto
Descripción
  • Diluent B 6 x 10
  • PKH26 cell linker in ethanol .5 mL

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Pictogramas

FlameExclamation mark
GHS02,GHS07

Palabra de señalización

Danger

Frases de peligro

H225,H319

Clasificaciones de peligro

Eye Irrit. 2 - Flam. Liq. 2

Código de clase de almacenamiento

3 - Flammable liquids

Punto de inflamabilidad (°F)

57.2 °F - closed cup

Punto de inflamabilidad (°C)

14.0 °C - closed cup

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Certificados de análisis (COA)

Lot/Batch Number
MKCK2680
SLBW5928
SLBV4711
SLBR8270V
SLBL6374V

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Hansang Cho et al.
Scientific reports, 3, 1823-1823 (2013-05-15)
Progressive microglial accumulation at amyloid-β (Aβ) plaques is a well-established signature of the pathology of Alzheimer's disease, but how and why microglia accumulate in the vicinity of Aβ plaques is unknown. To understand the distinct roles of Aβ on microglial
Hansang Cho et al.
Lab on a chip, 14(5), 972-978 (2014-01-17)
Neutrophils are the most abundant type of white blood cells in the circulation, protecting the body against pathogens and responding early to inflammation. Although we understand how neutrophils respond to individual stimuli, we know less about how they prioritize between
Jeffrey H Jennings et al.
American journal of respiratory cell and molecular biology, 32(2), 108-117 (2004-11-26)
Apoptotic cells must be cleared to resolve inflammation, but few resident alveolar macrophages (AMo) from normal lungs ingest apoptotic cells. We examined how Mo ingestion of apoptotic cells is altered during immune inflammation induced by intratracheal challenge of primed C57BL/6
Hiroshi Kanno et al.
International journal of molecular sciences, 14(5), 9604-9617 (2013-05-07)
A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported
Sung Hoon Baik et al.
Neurobiology of aging, 35(6), 1286-1292 (2014-02-04)
Immune responses in the brain are thought to play a role in disorders of the central nervous system, but an understanding of the process underlying how immune cells get into the brain and their fate there remains unclear. In this
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PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

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