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N5661

Sigma-Aldrich

Nuclease S1 from Aspergillus oryzae

for single-strand DNA/RNA digestion

Sinónimos:

Endonuclease S1

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About This Item

Número de CAS:
37288-25-8
Comisión internacional de enzimas:
3.1.30.1 (BRENDA, IUBMB)
MDL number:
MFCD00131010
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus sp. (A. oryzae)

Quality Level

200

form

solution

concentration

≥100000 units/mL

technique(s)

DNA purification: suitable

suitability

suitable for nucleic acid purification

application(s)

cell analysis

shipped in

wet ice

storage temp.

−20°C

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General description

The Nuclease S1 enzyme from Aspergillus oryzae has the ability to degrade single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides.

Application

Nuclease S1 from Aspergillus oryzae has been used in a study to assess a biochemical method for mapping mutational alterations in DNA. It has also been used in a study to investigate the DNA damage and repair in a γ-irradiated rat brain tumor.

Biochem/physiol Actions

Nuclease S1 isolated from Aspergillus oryzae exhibits endo- and exolytic hydrolytic activity for the phosphodiester bonds of single-stranded DNA and RNA yielding 5′-phosphomononucleotide and 5′-phosphooligonucleotide end-products. It is used to digest non-annealed polynucleotide tails and hairpin loops in RNA and DNA duplexes and can be used to convert superhelical DNA to the linear form.
SI nuclease from Aspergillus oryzae can generate double-stranded DNA breaks in response to DNA nicks or abasic sites.

Unit Definition

One unit will cause 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C.

Physical form

Solution containing 30 mM sodium acetate, 50 mM NaCl, 1 mM ZnCl2, 50% glycerol, 2 mg/ml protein

Solo componentes del kit

Referencia del producto
Descripción
  • 30mM Sodium acetate .25-.25 %
  • 50mM Sodium chloride .29 %
  • 1mM Zinc chloride .01 %
  • Glycerol 50 %
  • 2mg/mL Protein .2 %

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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T E Shenk et al.
Proceedings of the National Academy of Sciences of the United States of America, 72(3), 989-993 (1975-03-01)
S1 nuclease (EC 3.1.4.X), a single-strand-specific nuclease, can be used to accurately map the location of mutational alterations in simian virus 40 (SV40) DNA. Deletions of between 32 and 190 base pairs, which are at or below the limit of
S1 nuclease from Aspergillus oryzae for the detection of DNA damage and repair in the gamma-irradiated intracerebral rat gliosarcoma 9L.
P H Gutin et al.
Radiation research, 72(1), 100-106 (1977-10-01)
F Harada et al.
Nucleic acids research, 2(6), 865-871 (1975-06-01)
Nuclease S1 specifically hydrolizes tRNAs in their anticodon loops, forming new 5' phosphate and 3' OH ends. Some single-stranded regions are not cut by nuclease S1. The strong preference of nuclease S1 for the anticodon region can be used for
Marwan Hassani et al.
iScience, 24(8), 102913-102913 (2021-08-20)
Mepolizumab (anti-IL-5) is a successful biological for treatment of T2/eosinophilic asthma by blocking the IL-5-eosinophil axis. The kinetics of human eosinophils in blood and sputum was determined to better understand the underlying mechanism(s). Pulse-chase labeling was performed with 6,6-2H2-glucose in
M A Chaudhry et al.
Nucleic acids research, 23(19), 3805-3809 (1995-10-11)
Defined DNA substrates containing discrete abasic sites or paired abasic sites set 1, 3, 5 and 7 bases apart on opposite strands were constructed to examine the reactivity of S1, mung bean and P1 nucleases towards abasic sites. None of
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Protocolos

Nuclease S1 Enzymatic Assay

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

Nuclease S1 Enzymatic Assay

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

Nuclease S1 Enzymatic Assay

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

Nuclease S1 Enzymatic Assay

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

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