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Merck
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Documentos clave

M9163

Sigma-Aldrich

Medium 199

With Hanks′ salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

Sinónimos:

M199 Medium, TCM 199

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Nombre del producto

Medium 199, 10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Nivel de calidad

400

esterilidad

sterile-filtered

Formulario

liquid

concentración

10 ×

técnicas

cell culture | mammalian: suitable

impurezas

endotoxin, tested

componentes

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Descripción general

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Reconstitución

Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

suplemento

Referencia del producto
Descripción
Precios

también adquirido normalmente con este producto

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
RNBL9600
RNBL9041
RNBL3253
RNBL1442
RNBJ6536

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Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Contenido relacionado

Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Medium 199 (M199) Formulation

Discover the formulation for Medium 199 for cell culture applications, including vaccine development.

Questions

  1. Hi, if I have 10x Medium 199, how can I dilute it in order to have 1x and grow cells? 

    1 answer

    1. Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
      Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
      (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
      • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
      • While gently stirring the water, add 100 ml of 10X medium.
      • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
      • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
      • Bring medium to final volume with additional tissue culture grade water.
      • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
      • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C

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