LY0100
Lysozyme Activity Kit
sufficient for 100 tests
Sinónimos:
Lysozyme assay kit
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
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General description
The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.
Application
Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme, lysozyme purified from chicken egg white and lysozyme from pilocarpine-stimulated saliva.
Biochem/physiol Actions
Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.
Storage and Stability
The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Los clientes también vieron
Paula Chequer Gouveia Mól et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1044-1045, 17-23 (2017-01-09)
In this study, a supermacroporous polyacrylamide cryogel was produced by cryo-polymerization and activated with Tris(hydroxymethyl)aminomethane (Tris-cryogel) to be applied as an affinity ligand for a one step purification of lysozyme (LYZ), directly from chicken egg white (EW). The Tris-cryogel presented
Silica Nanopollens Enhance Adhesion for Long-Term Bacterial Inhibition.
Song, H., et al.
Journal of the American Chemical Society, 138, 6455-6462 (2016)
Modifications to the peptidoglycan backbone help bacteria to establish infection.
Davis K M and Jeffrey N W
Infection and Immunity, 799(2), 562-570 (2011)
Water-soluble allyl sulfones for dual site-specific labelling of proteins and cyclic peptides.
Wang T, et al.
Chemical Science, 7(5), 3234-3239 (2016)
Development of an affinity cryogel for one step purification of lysozyme from chicken egg white.
Mol P C G, et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1044, 17-23 (2017)
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