H7411

Anti-HA monoclonal

clone HA-7, purified from hybridoma cell culture

• Sinónimos: Anti-HA, Hemaglutinina anti-gripal
• MDL number: MFCD00803873
• UNSPSC Code: 12352203
• NACRES: NA.56

Propiedades

• biological source: mouse
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HA-7, monoclonal
• form: buffered aqueous solution
• storage condition: protect from light
• concentration: 1 mg/mL
• technique(s): immunocytochemistry: 1-5 μg/mL using HA tagged fusion protein transfected into mammalian cells fixed with paraformaldehyde.
• shipped in: wet ice
• storage temp.: −20°C

Descripción

General description

Anti HA-FITC is a mouse monoclonal antibody derived from the HA-7 hybridoma. It is isolated from ascites fluid and conjugated to fluorescein isothiocyanate, isomer I (FITC). The antibody recognizes the HA tag expressed on the N- or C-terminus on HA tagged H7411proteins. The antibody reacts specifically with HA tagged proteins by immunocytochemistry.

Hemagglutinin (HA) is the major envelope glycoprotein of the influenza virus, along with neuraminidase.

Specificity

El anticuerpo reconoce tanto las formas nativas como las desnaturalizadas-reducidas de las proteínas etiquetadas con HA y es reactivo con las proteínas de fusión etiquetadas con HA en N-terminal o C-terminal expresadas en E. coli o en células de mamífero.

Immunogen

péptido sintético correspondiente a un fragmento de la hemaglutinina (HA) del virus de la gripe humana, conocida como etiqueta-HA, conjugado con KLH

Application

Antibody suitable for Immunofluorescence. Recommended concentration 1-5 mg/ml. For immunocytochemistry, use HA tagged fusion protein transfected into mammalian cells, then fixed with paraformaldehyde. Anti-HA-FITC antibody, Mouse monoclonal has been used:

• in the labeling of HA-tagged transient receptor potential melastatin 7 (TRPM7) channel protein for immunocytochemistry studies in lymphoblast cell DT40 and human embryonic kidney cells (HEK-293) cells
• in western blotting detection of spindle-associated proteins in Trypanosoma brucei
• in immunostaining of vascular smooth muscle cells (SMCs) for immunocytofluorescence studies

Biochem/physiol Actions

Hemagglutinin (HA) induces immune response post viral infection. Fluorescein isothiocyanate (FITC) conjugated with the influenza virus HA is majorly used in intracellular tracking of the virus and also in flow cytometric studies.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable