The immunoglobulin concentration of this product is not measured. This is a pooled product from adult AB negative males. Based on information published in the Geigy Scientific Tables, the average immunoglobulin levels for adult males, ages 20 – 70, are as follows:
IgG
10.69 – 12.04 g./L
IgA
1.32 – 2.34 g/L
IgM (not age dependent)
0.77 g/L
H4522
Suero humano
from human male AB plasma, USA origin, sterile-filtered
Sinónimos:
plasma humano, suero humano, sueros humanos
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About This Item
origen biológico:
human male plasma (Type AB)
origen:
USA origin
esterilidad:
sterile-filtered
Condiciones de envío:
dry ice
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
human male plasma (Type AB)
Nivel de calidad
esterilidad
sterile-filtered
composición
hemoglobin, ≤30 mg/dL
origen
USA origin
técnicas
cell culture | mammalian: suitable
cell culture | stem cell: suitable
impurezas
≤10 EU/mL endotoxin
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
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Aplicación
El suero AB humano se utiliza en aplicaciones de ingeniería genética, trasplantes y terapia celular para la expansión de células madre mesenquimatosas (MSC) del tejido adiposo o las células del estroma mesenquimatoso de la médula ósea humana; para la generación y el trasplante de injertos normalizados de células madre del epitelio del limbo esclerocorneal; para la expansión ex vivo de células NK a partir de sangre periférica en medio de expansión de células madre hematopoyéticas; y para actualización de la tecnología de cultivo de islote humano pretrasplante.
El suero humano se ha utilizado como componente de la disolución salina tamponada con fosfato con detergente Tween® (PBST) en el enzimoinmunoanálisis de adsorción (ELISA) de doble captura, como muestra biológica simulada para evaluar el rendimiento del ELISA en papel con muestras biológicas complejas, como diluyente para crear una dilución en serie de la muestra basal para la generación de la curva de calibración para NISTmAb enriquecido en suero, así como para la muestra de paciente de mieloma múltiple (MM) MM2232
Acciones bioquímicas o fisiológicas
El suero AB humano se obtiene normalmente permitiendo la coagulación natural de sangre completa, lo que se conoce como suero «fuera del coágulo» (OTC). Se prefiere el suero humano obtenido de sangre completa coagulada, ya que en general contiene concentraciones mayores, y una gama más diversa, de factores de crecimiento para un crecimiento celular óptimo. El suero humano procedente de donantes de sangre de tipo AB carece de anticuerpos específicos para los antígenos de las sangres de tipo A y B. Esto lo convierte en un suero donante universal que es adecuado para varios tipos de cultivos celulares, entre ellas las líneas de células madre humanas.
Envase
El embalaje puede variar para diferentes lotes debido a la escasez de las botellas utilizadas para embalar este producto. La presencia o falta de envoltura retráctil alrededor de la tapa de la botella no afecta a la integridad/esterilidad del producto.
Advertencia
Se analizó cada donante para la hepatitis B y C y se observó que no eran reactivos , y tampoco lo eran para los anticuerpos del virus de la inmunodeficiencia humana (VIH) mediante ELISA. No obstante, los productos de origen humano deben considerarse potencialmente infecciosos y manejarse en consecuencia.
Nota de análisis
Analizada la presencia de endotoxinas
Otras notas
No es inusual que nuestros productos séricos humanos estén turbios. Este producto procede de la combinación de sueros y la turbidez indica que hay lípidos en el suero. No se dispone de productos séricos humanos transparentes, carentes de lípidos.
Información legal
TWEEN is a registered trademark of Croda International PLC
Cláusula de descargo de responsabilidad
SÓLO PARA INVESTIGACIÓN. Este producto está regulado en Francia cuando está destinado a ser utilizado con fines científicos, como para actividades de importación y exportación (artículo L 1211-1, apartado 2, del Código de Salud Pública). El comprador (es decir, el usuario final) está obligado a obtener una autorización de importación del Ministerio de Investigación de Francia referida al artículo L1245-5-1 II del Código de Salud Pública. Al pedir este producto, usted confirma que ha obtenido la autorización de importación adecuada.
Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Certificados de análisis (COA)
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Artículos
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
Learn about the impact of filtration on hematopoietic cell culture media containing vital T cell factors and other media components.
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What is the antibodies concentration in Product H4522, human serum?
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Il prodotto è stato testato per negatività a HSV?
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Each donor is tested for and found non-reactive to Hepatitis B & C and non-reactive to Human Immunodeficiency Virus (HIV) antibody by ELISA. HSV is not on the list of agents that are tested. Nevertheless, products of human origin should be considered potentially infectious and handled accordingly. See the link below to review a sample or lot specific Certificate of Analysis for this product: https://www.sigmaaldrich.com/product/sigma/i4506#product-documentation
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If this is "serum" derived from "plasma" the plasma would have contained anti-coagulant. To turn the plasma into serum it has to be clotted. Has this product been clotted to remove the fibrin? Or is it still in fact plasma?
1 answer-
The product has been clotted. Blood is collected and treated with citrate (anticoagulant) which inhibits clotting initially. The blood is then centrifuged. When the spin is complete, layers will appear which allow a differentiation between the cells and the plasma. The plasma is removed and calcium is added to activate the clotting cascade. The preparation is then centrifuged again and the result is a large clot which is pelleted to the bottom of the tube with a serum layer on top.
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Are Human Serum products H4522 and H6914 suitable for complement assays? Is there testing available for complement levels or activity?
1 answer-
Human Serum products H4522 and H6914 may not be suitable for complement assays when stored at -20°C due to potential degradation of complement activity. For complement assays, alternatives like S1764 (Complement sera human) and C9473 (Complement Serum Standard human) are recommended. It is advised to review the Certificates of Analysis (CoAs) for these products to ensure they meet the specific requirements of your complement assay
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Which human serum is recommend for a nucleic acid stability assay, specifically for incubating siRNA in serum to represent bloodstream kinetics?
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The use of human serum for a nucleic acid stability assay has not been performed as it is not part of our lab's product characterization focus. However, the citation titled "Improving gene silencing of siRNAs via tricyclo-DNA modification, PMID: 21687522" suggest that product H4522 may be suitable for this application. It is recommended to refer to the publication for additional insights.
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What are the differences between serum and plasma-derived serum?
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Whole blood serum is obtained by allowing natural clotting followed by filtration,lacking fibrinogen. Plasma-derived serum is obtained from non-clotted whole blood through centrifugation.
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What Biosafety Level would be suitable for human serum such as H4522, H5667, and H6914?
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Human origin products from normal donors should be used at Biosafety Level 2 (BSL-2), which is suitable for handling moderate-risk agents causing human disease. Thus, treat these products as potentially infectious and handle accordingly.
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what does this do
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Human AB serum is added as a supplement to culture media at a concentration of 2-10% to provide a mixture of nutritional, hormonal, growth, and attachment factors. The serum also acts as a buffer to the cell culture system against a variety of disruptions to cell growth and toxic effects such as pH change, proteolytic activity, or the presence of heavy metals or endotoxin.
Please see the link below for more information:
https://www.sigmaaldrich.com/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/serum-cell-tissue-faqHelpful?
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Is product H4522 obtained using Institutional Review Board (IRB)-approved consent forms and protocols?
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According to the supplier:
Each donor unit used in the preparation of this material was tested and found negative for HBsAG, HBV-NAT, HIV-1/2, HCV, HCV-NAT, HIV-1 NAT, and Syphilis per FDA regulations. The human plasma was collected from male donors' blood type AB in FDA licensed commercial donor establishments located in the USA in accordance with ethical standards. Each donor signed an informed consent form prior to collection, which is traceable by the unique identifiable number to maintain anonymity. A quality assurance system is in place to ensure traceability to individual donor. Documentation to this effect is available upon request.Helpful?
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Could you please confirm if the serum contains normal levels of human complement components?
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This product has not been treated to remove or inactivate complement components, however no testing has been performed to confirm these levels. It is a pooled product derived from 50 - 70 donors per lot. Any active complement be expected to meet 'normal levels.
Should complement testing be a requirement, please see product S1764. This is a lyophilized human serum product tested for CH50 activity. See the links below to review a sample Certificate of Analysis and the product datasheet:
S1764 Certificate of Analysis
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/S1764-1MLSLCQ7524.pdfS1764 Datasheet
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/408/363/s1764dat.pdfHelpful?
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