The recommended storage duration for Fibronectin (F0895) is up to 1 year at 4°C. There has been no testing done on its stability after storage at -80°C or -20°C. A diluted solution of 10ug/mL in PBS can be stored at 4°C for 2-4 weeks. However, the solution cannot be reused to coat culture ware surfaces multiple times; the recommended process involves coating surfaces with a minimal volume, allowing them to air dry for 45 minutes at room temperature, and then storing the cultureware at 4°C for 2-4 weeks.
F0895
Human Fibronectin
from human plasma, liquid, 1 mg/mL, suitable for cell culture
Sinónimos:
Fibronectina
About This Item
Productos recomendados
Nombre del producto
FIBRONECTINA DEL PLASMA HUMANO, liquid, 0.1% (Solution), BioReagent, suitable for cell culture
biological source
human plasma
Quality Level
sterility
sterile
product line
BioReagent
form
liquid
packaging
pkg of 1 mg
pkg of 2 mg
pkg of 5 mg
concentration
0.1% (Solution)
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
solubility
saline: 0.05 M (Tris-buffered)
UniProt accession no.
binding specificity
Peptide Source: Collagen
shipped in
wet ice
storage temp.
2-8°C
Gene Information
human ... FN1(2335)
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General description
La FN aparece como un dímero proteico, unido por dos enlaces disulfuro en el extremo C-terminal.[2] Dentro de la estructura de la fibronectina, los dominios están dispuestos de forma compacta cerca de los extremos amino y carboxilo terminales de las cadenas peptídicas.[3] Además, pese a proceder de un solo gen, en humanos se han identificado hasta 20 variantes distintas de este gen.[2]
Application
Biochem/physiol Actions
Features and Benefits
- Glucoproteína adhesiva
- Derivada del plasma humano
- Cobertura de superficie recomendada de 1-5 μg/cm2
Preparation Note
En el recubrimiento de las superficies de cultivo, la fibronectina debe diluirse en disolución salina estéril equilibrada y recubrirse con un volumen mínimo. Luego hay que dejar secar la superficie al aire durante 45 minutos a temperatura ambiente y puede conservarse durante 2-4 semanas a 2-8°C
Analysis Note
Legal Information
Disclaimer
related product
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artículos
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.
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What is the recommended storage duration for Fibronectin (F0895) at 4°C, -20°C, and -80°C? How long can a diluted solution of 10ug/mL in PBS be stored at 4°C? Is it possible to reuse the Fibronectin solution to coat culture ware surfaces multiple times?
1 answer-
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What is the recommended dilution rate for coating the culture surface with F0895-1MG Fibronectin as per the preparation instructions?
1 answer-
The recommended dilution rate for coating the culture surface with F0895-1MG Fibronectin, as per the preparation instructions, is to start with a concentration in the range of 1 - 5 ug/cm^2. For a 96-well plate well with a surface area of about 0.34 cm^2, approximately 2 ul of F0895 further diluted in saline with a total volume of around 50 ul would be sufficient to cover the bottom of the well.
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Is Product F0895, Fibronectin from human plasma, sterile?
1 answer-
Fibronectin 0.1% solution is a sterile product. It is tested for sterility. The sterility test results can be found on the lot-specific certificate of analysis.
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How much Product F0895, Fibronectin from human plasma, do I use for my application?
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Recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
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How should I store and handle Product F0895, Fibronectin from human plasma?
1 answer-
Recommended storage of this material is 2-8 °C. Vortexing, excessive agitation, repeated freezing and thawing of reconstituted fibronectin are not recommended.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the solvent for Product F0895, Fibronectin from human plasma?
1 answer-
This material is solubilized in a 0.05 M Tris-buffered saline, pH 7.5.
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