RAG

89040605, mouse kidney, Ameboid-like

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: RAG, 89040605
• origen biológico: mouse kidney
• modo de crecimiento: Adherent
• cariotipo: Modal no. 68, hypertriploid
• morfología: Ameboid-like
• productos: ES-2
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse BALB/c renal adenocarcinoma

Descripción línea celular

Derived from the original Renal-2a BALB/c renal adenocarcinoma. Cells produce the unique kidney specific esterase ES-2 and have been used in somatic cell hybridisation studies because of their azaguanine resistant properties.

Aplicación

Hybridisation, resistance and tumourigenicity studies

Medio de cultivo

EMEM (HBSS) + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Otras notas

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