SP2/O-Ag14 Cell Line murine

Non-producing hybridoma, 85072401

• Sinónimos: Mouse Cell Line
• Código UNSPSC: 12352207

Propiedades

• origen biológico: mouse spleen
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse x Mouse myeloma, non-producing

Descripción línea celular

Sp2/0-Ag14 is a non-Ig-secreting or synthesising line derived from a cell line created by fusing a BALB/c mouse spleen cell and the mouse myeloma P3X63Ag8. Resistant to 8-azaguanine at 20ug/ml and does not survive in HAT containing media. HAT sensitive mouse myeloma hybrid suitable for use as a hybridoma fusion partner - in this sense, functionally it is a myeloma cell line and often referred to as such in the literature.

Aplicación

Fusion partner

Medio de cultivo

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. HAT sensitive.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.