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Merck

A8094

Sigma-Aldrich

L-arginina

98.5-101.0%, suitable for cell culture, BioXtra, non-animal source

Sinónimos:

Ácido (S)-2-amino-5-guanidinopentanoico

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About This Item

Fórmula lineal:
H2NC(=NH)NH(CH2)3CH(NH2)CO2H
Número de CAS:
Peso molecular:
174.20
Beilstein/REAXYS Number:
1725413
EC Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.26
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Nombre del producto

L-arginina, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

biological source

non-animal source

Quality Level

agency

USP/NF
meets EP testing specifications
meets USP testing specifications

product line

BioXtra

assay

98.5-101.0%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

222 °C (dec.) (lit.)

solubility

H2O: 100 mg/mL

application(s)

agriculture
peptide synthesis
pharmaceutical (small molecule)

SMILES string

N[C@@H](CCCNC(N)=N)C(O)=O

InChI

1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m0/s1

InChI key

ODKSFYDXXFIFQN-BYPYZUCNSA-N

Gene Information

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Application

L-Arginine has been used:
  • as a Roswell park memorial institute medium (RPMI) media component in the isolation and culture of peripheral blood mononuclear cells (PBMCs)[1]
  • as a RPMI media component for tissue culture[2]
  • in DMEM medium for the identification and quantification of phosphorylation sites by stable isotope labeling by amino acids in cell culture (SILAC) and LCMS/MS[3]

Biochem/physiol Actions

Sustrato de la óxido nítrico sintasa, que se convierte en citrulina y óxido nítrico (NO). Induce la liberación de insulina mediante un mecanismo dependiente del óxido nítrico.
L-Arginine is a conditionally essential amino acid, that play a vital role in the production of nitric oxide. It acts as a precursor for the synthesis of polyamines, agmatine and urea. L-Arginine is usually essential in children and non-essential in adults.[4]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

Requirement of the mTOR Kinase for the Regulation of Maf1 Phosphorylation and Control of RNA Polymerase III-dependent Transcription in Cancer Cells
Boris Shor
The Journal of Biological Chemistry, 285(20), 15380-15392 (2010)
Association of reactive oxygen and nitrogen intermediate and complement levels with apoptosis of peripheral blood mononuclear cells in lupus patients
Oates J C, et al.
Arthritis and Rheumatism, 56(11), 3738-3747 (2007)
J. P. Felix D'Mello
Amino Acids in Human Nutrition and Health, 225-225 (2012)
Cell-specific Labeling Enzymes for Analysis of Cell?Cell Communication in Continuous Co-culture
Tape CJ, et.al.
Molecular and Cellular Proteomics, 13(7), 1866-1876 (2014)
Anti-aging effects of L-arginine
Gad M Z
Journal of Advanced Research, 1(3), 169-177 (2010)

Protocolos

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

Protocolo del ensayo con MTT para medir la viabilidad, la proliferación y la citotoxicidad celulares. Instrucciones para la preparación de los reactivos MTT y ejemplos de aplicaciones.

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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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