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Merck

A3809

Sigma-Aldrich

Asparaginase from Escherichia coli

lyophilized powder, 100-300 units/mg protein (biuret)

Sinónimos:

L-Asparagine Amidohydrolase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
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Formulario

lyophilized powder

Nivel de calidad

actividad específica

100-300 units/mg protein (biuret)

composición

Protein, ≥60%

temp. de almacenamiento

2-8°C

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Descripción general

Asparaginase is a bacterial enzyme[1] and also a chemotherapeutic drug.[2]

Aplicación

Asparaginase from Escherichia coli has been used:
  • to compare the cytotoxic effect of L-asparaginase purified from Streptomyces brollosae NEAE-115[3]
  • as a standard in asparaginase assay to quantify asparaginase activities in various eel tissues[4]
  • to elicit amino acid deprivation[5]

Acciones bioquímicas o fisiológicas

Asparaginase (ASNase) products are usually obtained from Escherichia coli and Erwinia chrysanthemi. These enzymes can block the synthesis of protein in tumor cells. It shows high activity in the G1 phase of the cell cycle.[6] It is capable of causing pancreatitis in leukemia patients.[2]
Asparaginase is used in enzymatic assays and to convert asparagine to aspartic acid. Asparaginase is used to reduce the formation of acrylamide in starchy food products. It is also used as a chemotherapy agent for acute lymphoblastic leukemia [7]. Product A3809 is from Escherichia coli and is provided as a lyophilized powder containing sodium chloride.
Asparaginase may cause cell death in leukemic cells by converting the necessary L-asparagine to aspartic acid and ammonia [7]. Asparaginase is allosterically regulated and is crucial for proper cell functioning [8].

Definición de unidad

One unit will liberate 1.0 μmole of ammonia from L-asparagine per min at pH 8.6 at 37 °C.

Forma física

Lyophilized powder containing sodium chloride

Nota de preparación

Chromatographically purified

Nota de análisis

Protein determined by biuret.

Pictogramas

Health hazardExclamation mark

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Repr. 2 - Skin Sens. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Certificados de análisis (COA)

Lot/Batch Number

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Visite la Librería de documentos

Jie Xiong et al.
EBioMedicine, 72, 103614-103614 (2021-10-11)
Metabolic reprogramming plays an essential role on lymphoma progression. Dysregulation of glutamine metabolism is implicated in natural-killer T-cell lymphoma (NKTCL) and tumor cell response to asparaginase-based anti-metabolic treatment. To understand the metabolomic alterations and determine the potential therapeutic target of
Asparagine Synthetase Is Highly Expressed at Baseline in the Pancreas Through Heightened PERK Signaling
Mukherjee A, et al.
Cellular and molecular gastroenterology and hepatology (2019)
Purification, characterization and immunogenicity assessment of glutaminase free L-asparaginase from Streptomyces brollosae NEAE-115
El-Naggar NEA, et al.
BMC Pharmacology & Toxicology, 19(1), 1-15 (2018)
Oxidized phospholipids regulate amino acid metabolism through MTHFD2 to facilitate nucleotide release in endothelial cells
Hitzel J, et al.
Nature Communications, 9(2292), 1-18 (2018)
Mi-Kyung Yun et al.
Journal of molecular biology, 369(3), 794-811 (2007-04-25)
AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Analytical ultracentifugation and X-ray crystallography reveal that AnsA forms a tetrameric structure as a dimer of two intimate dimers. Kinetic analysis of the enzyme reveals that AnsA

Questions

1–7 of 7 Questions  
  1. What is the actual quantity (in the gram) in 1 unite of the enzyme. Because, we want use the enzyme in gram. such as i have 300 unit of this enzyme. So please tell what is the exact quantity in gram

    1 answer
    1. The activity of this product varies from lot to lot with the minimum amount in a 1000 unit vial being 3.33 mg. This is based on the maximum allowable activity of 300 units per milligram protein and the maximum 100% protein. The maximum amount will be 16 mg per 1000 unit vial. This is based on the minimum allowable activity of 100 units per milligram protein and the minimum allowable protein content of 60%. Please see the link below to access a sample or lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/a3809#product-documentation

      Helpful?

  2. I would like to obtain the asparaginase (A3809-1KU) enzymatic assay protocol.

    1 answer
    1. Helpful?

  3. How does the storage temperature relate to shipping conditions?

    1 answer
    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  4. What is the molecular weight of this enzyme (Product No. A3809)?

    1 answer
    1. This enzyme consists of a tetramer composed of four identical subunits of 34,080 Daltons each. The molecular weight of the tetramer is 136,320 Daltons.

      Helpful?

  5. How should this enzyme (Product No. A3809) be reconstituted?

    1 answer
    1. Prior to determining the activity of this enzyme, Sigma reconstitutes it directly in deionized water. Solutions should be prepared fresh, as the stability of stock solutions has not been evaluated.

      Helpful?

  6. What is the source of this enzyme (Product No. A3809)?

    1 answer
    1. This enzyme is obtained from a natural extraction of E. coli. It is not from a recombinant source.

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  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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