This product ships at ambient temperature and is stable for relatively short periods at room temperature. There is a 1 - 2 week allowance for transit time. A storage temperature excursion of 2 days is not expected to impact the quality or performance of this material.
10771
Histopaque®-1077
Histopaque®-1077
sterile-filtered, density: 1.077
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.75
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
esterilidad
sterile-filtered
Nivel de calidad
Formulario
liquid
caducidad
Expiry date on the label.
técnicas
cell culture | mammalian: suitable
impurezas
endotoxin, tested
densidad
1.077
aplicaciones
hematology
histology
temp. de almacenamiento
2-8°C
Descripción general
Histopaque-1077 es un medio estéril, probado para endotoxinas y listo para usar. La composición de Histopaque consiste en polisacarosa y diatrizoato de sodio, ajustados a una densidad de 1,077 g/ml. Este medio listo para usar facilita la recuperación rápida de los linfocitos viables y otras células mononucleares de pequeños volúmenes de sangre completa o médula ósea.
Aplicación
Histopaque-1077 se ha utilizado para:
- la determinación de los antígenos del complejo principal de histocompatibilidad (HLA)
- como etapa inicial del aislamiento antes del recuento de linfocitos T, B y ‘nulos’.
- preparación de suspensiones puras de linfocitos para cultivos celulares y ensayos de citotoxicidad.
- Aislamiento y purificación de células mononucleares de sangre periférica (PBMC) y células mononucleares de médula ósea (BMMC)[1]
- aislamiento de linfocitos humanos[2]
- aislamiento de células tumorales circulantes de cáncer de mama (CTC) en sangre completa[3]
- Histopaque-1077 se utiliza junto con Histopaque-1119 ya que facilita la recuperación rápida de células mononucleares viables y granulocitos de pequeños volúmenes de sangre completa
Características y beneficios
Histopaque®-1077 ofrece numerosas ventajas, como:
- Recuperación óptima de las células viables
- Permite la separación selectiva de los tipos de células sanguíneas
- Previene la distorsión celular
- Preserva la viabilidad celular
- Interferencia mínima de células extrañas
- Rendimiento reproducible de lote a lote
- Esterilizado mediante filtración y probado para endotoxinas
- Estable a 2-8°C durante al menos 2 años cuando está protegido de la luz
Principio
La sangre anticoagulada se coloca en capas sobre el Histopaque-1077. Durante la centrifugación, la polisacarosa produce agregación de los eritrocitos, que sedimentan rápidamente. Los granulocitos se vuelven ligeramente hipertónicos, lo que aumenta su velocidad de sedimentación y provoca su depósito en el fondo del tubo de centrífuga. Los linfocitos y otras células mononucleares permanecen en la interfaz plasma/Histopaque-1077.
Información legal
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Opcional
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Referencia del producto
Descripción
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también adquirido normalmente con este producto
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1 - Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 3
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Artículos
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Protocolos
This troubleshooting guide addresses the most common sources of error observed when using Histopaque but is not meant to be a comprehensive list.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.
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Hello, we had a problem with the refrigerator leading the temperature increasing to 44 degrees Celsius for over the 2 days of weekend, so can our Histopaque-1077 stored in this refrigerator can be used anymore?
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Can Histopaque 10771 be utilized to separate tumor cells from monocytes?
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Histopaque 10771 is unlikely to be suitable for separating tumor cells from monocytes. It is designed to separate monocytes and lymphocytes from human blood. Unless the density of your specific tumor cells significantly differs from 1.077 g/ml, there is a possibility that tumor cells may be collected within the mononuclear cell band. Alternatives such as Percoll, Percoll Plus, or OptiPrep could be better options.
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If Histopaque-1077 is brought to room temperature, how long can it be left at that temperature before use? Specifically, will leaving it for a few hours cause any issues?
1 answer-
Ficoll Paque has a storage temperature range of 4°C to 30°C for unopened bottles, while opened bottles should be stored at 4°C to 8°C. However, Histopaque can be stored at room temperature for short periods.
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Can we use a ratio of blood to separation media of 4:3 with Histopaque-1077? Also, can Histopaque-1077 be used to dilute the blood up to 1:2 before layering with separation media with HBSS buffer, and then wash the cells too.
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Histopaque 10771/H8889 and Ficoll Paque solutions are produced with similar reagents. As Histopaque has a slightly lower density range, yields might be slightly lower. Therefore, it is a mixed bag and yields with Histopaque might be slightly lower. When observing a very high white cell count, dilution is recommended. The standard dilution for peripheral blood is generally 1:2. For bone marrow, a 1:2 or 1:4 dilution are more commonly used. The expectation is that cell recovery should be almost the same.
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Do you have a recommended protocol for purifying PBMCs from larger volumes using Histopaque 1077 in 50 mL tubes instead of 15 mL tubes?
1 answer-
For PBMCs, the catalog numbers for Histopaque 10771, H8889, and 10831 are applicable. The Instructions For Use indicate that a 15 ml centrifuge tube with 3 ml of Histopaque 10771 is recommended. This procedure can also be used for a 50 ml centrifuge tube, considering that the height of both tubes is comparable while their diameter differs. To accommodate a 50 ml centrifuge tube, it is advised to use 15 ml of Histopaque 10771. No alterations are needed for the centrifugation time and washing steps. It is sufficient to wash the isolated cells with isotonic PBS or cell culture media.
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Are Histopaque 1077 and Ficoll Paque pure products? What is the percentage of polysaccharide or polysucrose in Histopaque 1077 and Ficoll Paque?
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Both Histopaque and Ficoll Paque are formulated with a combination of polysucrose 400 and sodium diatrizoate. On average, Histopaque and Ficoll Paque solutions contain approximately 5.7% polysucrose and 9.0% sodium diatrizoate. The final density of these solutions is expected to be around 1.077 g/ml for both products with a typical density variation of +/- 0.001%.
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Can histopaque-1077 be used to isolate live tumor cells in suspension from dead cells/cell debris?
1 answer-
Histopaque 10771 is primarily intended for isolating mononuclear cells from human blood and bone marrow. It is also utilized in cell culture to eliminate dying cells from hybridomas. However, the effectiveness of Histopaque 10771 or Histopaque 10831 in purifying live cells from deceased liver cells remains untested. Percoll and OptiPrep are common alternative methods.
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Is Histopaque 10771 the recommended density for isolating or separating PBMCs in llama blood?
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Yes, Histopaque 1077 (catalog number 10771) is the recommended density for mammalian cells.
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Will Histopaque 10771 be suitable for isolating CD45-positive lymphocytes from mouse whole blood?
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While Histopaque 10771 can be used for isolating mouse mononuclear cells including lymphocytes and monocytes, the formulation may not be optimal. Alternatively, Histopaque 10831 is available, with a density of 1.083 g/ml and Ficoll Paque 1084 with a density of 1.084 g/mL. These formulations are specifically designed for the purification of mononuclear cells in rats, mice, and small mammals.
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What is the efficacy and reliability of using Histopaque®-1077 for separating low-density neutrophils (LDN) and normal-density neutrophils (NDN) from high-density neutrophils?
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The Histopaque 1077 procedure is not designed to purify low-density or high-density neutrophils. Instead, Histopaque 10771 is intended for purifying Peripheral Blood Mononuclear Cells (PBMCs). When using Histopaque 10771, it is expected that the collected mononuclear cells would contain 5% or less of neutrophils, likely in the form of low-density neutrophils.
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