A1682

Anti-Mouse IgG (Fab specific)–Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• Sinónimos: Goat Anti-Mouse IgG (Fab specific)–AP
• Número MDL: MFCD00162620
• Código UNSPSC: 12352203
• NACRES: NA.46

Propiedades

• origen biológico: goat
• Nivel de calidad: 200
• conjugado: alkaline phosphatase conjugate
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: secondary antibodies
• clon: polyclonal
• Formulario: buffered aqueous solution
• reactividad de especies: mouse
• no debe reaccionar con: human, rat
• técnicas: direct ELISA: 1:40,000; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10; western blot: 1:200,000-1:400.000 using assay detecting β-Actin in total cell extract of HeLa cells (5-10 μg per well)
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody detects mouse IgG (whole molecule). When tested by ELISA, the product reacts with the Fab fragment of mouse IgG but does not recognize the Fc fragment of mouse IgG, human IgG, IgA, IgM or rat IgG.

Inmunógeno

Purified mouse IgG

Aplicación

Alkaline phosphatase-conjugated goat anti-mouse IgG (Fab specific) was used as the secondary antibody in ELISA assays to measure the reacitivity of mABs to thyrogobulin peptides. Absorbance was measured at 405 nm.

Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody is suitable for use in western blot at 1:1000 dilution using human airway epithelial cells or using whole-cell extracts of rat tubal tissues . The antibody can also be used for immunohistochemistry (1:10 using formalin-fixed, paraffin-embedded sections).

Goat anti-mouse Fab specific alkaline phosphatase antibody was used to analyze serum Ig titers by His Sorb ELISA at 1:10000 at room temperature for two hours. Antibodies were diluted in PBS plus 0.2% BSA to reduce background.

Otras notas

Antibody adsorbed with human IgG and rat serum proteins.

Forma física

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ with 50% glycerol as stabilizer and 15 mM sodium azide

Nota de preparación

Adsorbed to reduce background staining with human or rat samples.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)