71183

Sodium acetate

anhydrous, BioUltra, for luminescence, for molecular biology, ≥99.0% (NT)

• Sinónimos: Sodium acetate anhydrous, Acetic acid Sodium salt anhydrous, Acetic acid sodium salt
• Fórmula lineal: CH₃COONa
• Número de CAS: 127-09-3
• Peso molecular: 82.03
• Beilstein: 3595639
• Número CE: 204-823-8
• Número MDL: MFCD00012459
• Código UNSPSC: 12161700
• eCl@ss: 39021906
• ID de la sustancia en PubChem: 57652226
• NACRES: NA.25

Propiedades

• grado: for luminescence; for molecular biology
• Línea del producto: BioUltra
• Ensayo: ≥99.0% (NT)
• Formulario: solid
• temp. de autoignición: 1112 °F
• impurezas: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pérdida: ≤0.5% loss on drying, 150 °C
• pH: 8.5-10.0 (25 °C, 3 M in H₂O)
• pKa: 4.76 (acetic acid)
• mp: >300 °C (dec.) (lit.)
• solubilidad: H₂O: 3 M at 20 °C, clear, colorless
• trazas de anión: chloride (Cl^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg; sulfate (SO₄^2-): ≤20 mg/kg
• trazas de catión: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤10 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤200 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤10 mg/kg; Zn: ≤5 mg/kg
• cadena SMILES: [Na+].CC([O-])=O
• λ: 3 M in H₂O
• Absorción UV: λ: 260 nm A_max: 0.03; λ: 280 nm A_max: 0.02
• InChI: 1S/C2H4O2.Na/c1-2(3)4;/h1H3,(H,3,4);/q;+1/p-1
• Clave InChI: VMHLLURERBWHNL-UHFFFAOYSA-M

Descripción

Aplicación

Sodium acetate is suitable for use as a buffer in:

• protein crystallization
• staining of gels in protein gel, electrophoresis, neutralization buffer for chromatography
• the preparation of standard (acetylcholine chloride) for hestrin assay
• substrate screening characterization for microfluidic microbial fuel cell array

Acciones bioquímicas o fisiológicas

Sodium acetate anhydrous is commonly used in molecular biology applications. This serves as a buffering agent in the DNA digestion experiments by ensuring the reaction has optimum pH. In general sodium acetate is preferred in purification, precipitation, crystallization, and other applications which require tight control of elemental content. Sodium acetate is extensively used as a retardant in plastics manufacturing, as a mordant in dyeing, and in the tanning of leather.

Otras notas

Extraction buffer component in the large-scale phenol extraction of RNA (for mRNA isolation).

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)