The long-term stability of this product in solution has not been determined. However, a similar Collagenase type IV product, C5138, notes that solutions are stable for several months when stored in aliquots at -20°C. Please see the link below to review the datasheet of a similar item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/380/876/c5138pis.pdf
C4-BIOC
Collagenase Type IV, Cls IV
Sinónimos:
Collagen A, Type IV Gelatinase
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About This Item
Código UNSPSC:
12352204
NACRES:
NA.78
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Descripción general
Collagenase Type IV, Cls IV is a member of the mammalian extracellular neutral metalloproteinases family. It digests type IV collagen, which is a major structural constituent of the basement membrane.[1]
This product is identical to Biochrom GmbH part numbers C4-22 and C4-28. Enzyme blending from collagenase, clostripain, with tryptic and proteolytic activities. Recommended for the isolation of Islets of Langerhans. Specific activity is 125 to 250 Mandl units per milligram of powdered substance.
Aplicación
Collagenase Type IV, Cls IV may be used:
- as a component in Dulbecco′s modified Eagle′s medium (DMEM)/F12 to digest endometrial samples isolated from in vitro fertilization (IVF) patients[2]
- for collagenase perfusion to isolate hepatocytes using collagenase-liver perfusion method in rats[3]
- in the isolation of rat glomerular endothelial cells (GEndCs)[4]
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Mingtai Gui et al.
Experimental and therapeutic medicine, 22(6), 1446-1446 (2021-11-02)
Continuous and irreversible cardiac hypertrophy can induce cardiac maladaptation and cardiac remodeling, resulting in increased risk of developing cardiovascular diseases. The present study was conducted to investigate the therapeutic effect of Huoxue Qianyang Qutan recipe (HQQR) on angiotensin II (Ang
P Huhtala et al.
The Journal of biological chemistry, 265(19), 11077-11082 (1990-07-05)
The structure of the gene for human 70-kDa type IV collagenase (gelatinase) was determined. Three overlapping genomic clones were isolated and shown to contain 0.4 kilobase (kb) of the 5'-flanking region, the 27-kb structural gene, and 4.5 kb of the
Hyun-Jeong Cho et al.
Biological & pharmaceutical bulletin, 28(8), 1418-1423 (2005-08-05)
Microsomal triglyceride (TG) transfer protein (MTP) is involved in the secretion of TG-rich very low-density lipoprotein (VLDL), a process which leads to the generation of hypertriglyceridemia and atherosclerosis. We investigated the possible role of Ca(2+) on MTP activity in hepatocytes.
Sina M Schalbetter et al.
Science advances, 8(9), eabi6672-eabi6672 (2022-03-03)
The prefrontal cortex (PFC) is a cortical brain region that regulates various cognitive functions. One distinctive feature of the PFC is its protracted adolescent maturation, which is necessary for acquiring mature cognitive abilities in adulthood. Here, we show that microglia
Michael Zeisberg et al.
Biochemical and biophysical research communications, 295(2), 401-407 (2002-08-02)
Type IV collagen, which is encoded by six genetically distinct alpha-chains (alpha 1-alpha 6), is a major component of the kidney glomerulus. The alpha 1(IV) and alpha 2(IV) chains are present predominantly in the mesangial matrix, whereas the alpha 3(IV)
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What is the storage condition and shelf life after reconstituting the product?
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How does one solubilize collagenase, and what is the usage concentration for digestion and cell isolation?
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The substance is soluble at 50mg/ml and can be dissolved in water, although it is recommended to use saline, NaCl, DPBS, Hanks, or a culture medium. It is worth noting that in peer-reviewed papers at sial.com, glomerular endothelial cells were isolated at 0.1% in DMEM.
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