30181

Fluorescein sodium salt

analytical standard

• Sinónimos: Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine
• Fórmula empírica (notación de Hill): C₂₀H₁₀Na₂O₅
• Número de CAS: 518-47-8
• Peso molecular: 376.27
• Número de índice de color: 45350
• Beilstein: 3833041
• Número CE: 208-253-0
• Número MDL: MFCD00167039
• Código UNSPSC: 85151701
• ID de la sustancia en PubChem: 329753341
• NACRES: NA.24

Propiedades

• grado: analytical standard
• Nivel de calidad: 100
• Ensayo: ≥97.5% (HPLC)
• caducidad: limited shelf life, expiry date on the label
• técnicas: HPLC: suitable; gas chromatography (GC): suitable
• impurezas: ≤15% water
• fluorescencia: λ_ex 460 nm; λ_em 515 nm(lit.)
• Formato: neat
• cadena SMILES: [Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1
• InChI: 1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
• Clave InChI: RGPLGPBQJOQFJS-UHFFFAOYSA-L

Descripción

Descripción general

Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11.[1] Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures.[2]

Aplicación

Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry.[3] It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM).[4]
It may also be used as an analytical reference standard for the quantification of the analyte in the following:

• Soil samples using high-performance liquid chromatography (HPLC).[2]
• Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE).[5]

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Nota de análisis

remarks on ICP: Na positive

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): 423.7 °F - Pensky-Martens closed cup
• Punto de inflamabilidad (°C): 217.6 °C - Pensky-Martens closed cup