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UFC9050

Millipore

Filtro de ultracentrífuga Amicon®, MWCO 50 kDa

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 50 kDa

Sinónimos:

Concentrador para centrífuga, MWCO de 50 kDa, volumen de muestra 15 ml

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About This Item

Código UNSPSC:
41104916
eCl@ss:
36100101
NACRES:
NB.24
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Materiales

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Nivel de calidad

400

esterilidad

non-sterile

Línea del producto

Amicon®

fabricante / nombre comercial

Amicon® Ultra

sustainability

Greener Alternative Product

Parámetros

15 mL sample volume

técnicas

protein extraction: suitable
protein purification: suitable

Longitud

12.1 cm (4.807 in.)

Diámetro

2.97 cm

filtración área

7.6 cm2

volumen

15 mL

volumen de trabajo

15 mL

tamaño de poro

50 kDa MWCO

categoría alternativa más sostenible

Re-engineered

Condiciones de envío

ambient

Descripción general

Las unidades de centrífuga Amicon® Ultra de 15 ml con filtros Ultracel® de corte de peso molecular de 50 000 Dalton proporcionan una ultrafiltración rápida y una retención fiable de las macromoléculas. Por ejemplo, la seroalbúmina bovina (67 kDa) a una concentración inicial de 1 mg/ml es retenida al >95 % .

El diseño vertical y la superficie de membrana disponible proporcionan un procesamiento rápido de la muestra, una elevada recuperación de la muestra (normalmente superior al 90 % de la disolución inicial diluida) y la capacidad de concentración de 80 veces. La polarización del soluto y el consiguiente ensuciamiento de la membrana se reducen al mínimo gracias al diseño vertical, y un bloqueo físico del filtro evita la centrifugación hasta la sequedad y la posible pérdida de muestra.

El concentrado se recoge del depósito de muestras de la unidad de filtración utilizando una pipeta, mientras que el ultrafiltrado se recoge en el tubo de centrífuga suministrado. La unidad puede centrifugarse en un rotor oscilante o en un rotor de ángulo fijo. Con su diseño de membrana vertical y el bloqueo, los filtros Amicon®Ultra de 15 ml ofrecen la mayor recuperación con tiempos de centrifugado cortos.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

Aplicación

  • Ultrafiltración con membrana Ultracel® de corte de peso molecular de 50 000 dalton.
  • Concentración de muestras biológicas que contienen antígenos, anticuerpos, enzimas, ácidos nucleicos o microorganismos.
  • Purificación de los componentes macromoleculares encontrados en extractos de cultivo tisular o lisados celulares y eliminación de proteínas antes de la HPLS.
  • Concentración de las proteínas diluidas o prepurificadas de los eluyentes de la columna.
  • Desalación, cambio de disoluciones tampón y diálisis de proteínas.

Características y beneficios

  • La membrana de celulosa regenerada Ultracel® con un NMWCO de 50 kDa proporciona una recuperación de retenido superior al 90 %.
  • La membrana vertical reduce la polarización de la concentración para tiempos de centrifugado ultrarrápidos (de hasta 10 – 15 minutos).
  • La membrana termosellada minimiza los extraíbles posteriores.
  • Integridad probada al 100 % para un rendimiento fiable.
  • Cómoda supervisión de la muestra con carcasa traslúcida y gradaciones de volumen.
  • El acceso directo a la muestra con pipeta elimina la etapa de procesamiento para recuperar el concentrado.
  • Factores de concentración elevada de X80 – 100.

Forma física

Código de colores: Transparente con tapa rosa

Información legal

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10-13 - German Storage Class 10 to 13

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

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Nanoparticle Ultrafiltration

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Nanoparticle Ultrafiltration

Filtration methods and membrane filter selection in nanoparticle purification and production.

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Ver todo

Questions

1–4 of 4 Questions  

  1. Can this filter system block large molecules like lipids, proteins, sugars, or starch and allow small molecules to pass through like water, ethyl alcohol, ethyl acetate, and vanillin.

    1 answer

    1. The Amicon Ultra products are designed to retain, not filter, and allow high recovery of smaller molecules. For example, let's say you have a 120 kDa molecule and you use a 50 kDa mwco filter in the Amicon Ultra. Most large molecules will be recovered, even 90-98%. However, depending on the size of a small molecule, it is hard to determine if 20-80% of something smaller will pass through. Shape matters and size matters. In this example, if you had a 1 kDa small molecule and a 20 kDa, one may expect more of the 1 kDa to pass through and less of the 20 kDa, if any. One key can sometimes be to spin slower and longer. By doing this, more of the small may pass through.

      Helpful?

  2. Can product UFC505008, Amicon® Ultra Centrifugal Filter, 50 kDa MWCO effectively concentrate the AAV virus sample ? Is it recommended to block the membrane before adding the sample as AAV strongly adhere to the column?

    1 answer

    1. Yes, product UFC505008 ,Amicon Ultra Centrifugal filter is suitable for concentrating the AAV virus up to a certain point. However, to optimize the process and prevent aggregate formation, it is recommended to use larger volume Amicon Ultras (15 ml) and avoid over-concentration.

      Helpful?

  3. My antibody solution contains 20% glycerol. Can I use this poduct to get rid of the glycerol? which dialysis buffer should be used?

    1 answer

    1. Yes, Amicon products that have a 10 kDa NMWCO pore size are suitable for the removal of glycerol from antibody preparations. The protocol below was provided by one of our antibody suppliers - Atlas Antibodies. Note that this protocol is written for the 0.5 mL product UFC501096, Volumes and possibly spin times would have to be adjusted to accommodate the 15 mL Amicon products.

      Glycerol can be removed using Amicon Centrifugal Filter Units, 10 kDa cutoff (Merck Millipore UFC501096).
      The filter unit concentrates the antibody sample during centrifugation by allowing the buffer to flow through the membrane but stops any proteins above 10 kDa from passing.

      The antibody is then resuspended in a buffer of choice, thus allowing for buffer exchange. Consider testing the antibody +/- the glycerol cleanup, to see whether there is an improvement. Some antibodies perform better even if there is glycerol in the buffer.

      PROCEDURE
      1. Dilute the antibody to 400 μl with sterile PBS (or other buffer of choice).
      2. Place the Amicon filter unit into the supplied collection tube.
      3. Add 400 μl of diluted antibody to the filter unit. Close the lid.
      4. Spin for 5-10 minutes at 14k*g or until 100 μl of the antibody sample remains in the spin column. If this has not been achieved during the first 5 min, then centrifuge for a few more minutes until only 100 μl remains.
      Please note that the suggested time it takes to concentrate the sample down to 100 μl should be seen only as a guideline and will ultimately have to be determined by the user. For all spins, place the filter part of the filter unit and the elbow of the collection tube towards the centre of the rotor. All spins are performed at room temperature.
      5. Discard the flow through.
      6. Add 300 μl of sterile PBS (or other buffer of choice) to the filter unit and centrifuge again as outlined in step 4. Then discard the flow through.
      7. Repeat step 6 four more times until the glycerol content is 0,02 %. 5-minute centrifugations are usually enough to reach 100 μl left in the filter unit at these steps. In the last round, centrifuge for 4 minutes until 100 μl is reached.
      8. In new collection tube, place spin column upside down.
      9. Spin for 2 min at 1k*g to collect buffer-exchanged antibody.

      Helpful?

  4. Hi , what simliar items do you have for this? I need 15ml centrifugal filters 50kda

    1 answer

    1. Unfortunately, this is the only 15mL centrifugal filter with 50kDa MWCO offered. There are other products available at different MWCO or volumes such as the Corning Spin-X UF products, see CLS431490 (20 mL).

      To discuss other options, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the required information so that a member of the Technical Service team can reach out to assist further. Thank you.

      Helpful?

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