MABS2321

Anti-Biotin Antibody, clone 8H6.8

• Sinónimos: Vitamin B7
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: 8H6.8, monoclonal
• purificado por: using protein G
• reactividad de especies (predicha por homología): all
• envase: antibody small pack of 100
• técnicas: ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• isotipo: IgG1κ
• Nº de acceso UniProt: n/a
• temp. de almacenamiento: 2-8°C

Descripción

Especificidad

Clone 8H6.8 is a mouse monoclonal antibody that detects biotin-conjugated proteins.

Inmunógeno

Biotin conjugated to KLH.

Aplicación

Quality Control Testing

Evaluated by ELISA with Biotin-BSA.

ELISA Analysis: Serial dilutions from a representative lot detected Biotin-BSA in ELISA application.

Tested Applications

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Biotin-BSA.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected Biotin-conjugates in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Biotin, a bicyclic compound, is a water-soluble vitamin with a ureido (N-CO-N) and a tetrahydrothiophene ring. The ureido group is involved in its binding to avidin. The tetrahydrothiophene group is a valeric acid moiety. Although biotin molecule can exist in eight stereoisomers, the D-biotin isomer is the only one with biological activity. It plays important roles in a variety of critical metabolic reactions in the cell, and thus, is essential for normal human health, growth, and development. Its deficiency, particularly during pregnancy, leads to embryonic growth retardation, congenital malformation, and death. In mammals, biotin serves as a cofactor for four carboxylases that catalyze the transfer of a carboxyl group to targeted substrates. The four biotin-dependent carboxylases are: pyruvate carboxylase; propionly-CoA carboxylase; β-methylcrotonyl-CoA carboxylase; and acetyl-CoA carboxylase. These carboxylases play a critical role in the intermediate metabolism of gluconeogenesis, fatty acid synthesis, and amino acid catabolism. It is also reported to play a role in normal immune functions, including the production of antibodies, normal macrophage function, and the differentiation of T and B lymphocytes. This monoclonal antibody recognizes biotin conjugated to various proteins in ELISA and other immunochemical applications. (Ref.: Said, SM. (2012). Subcell. Biochem. 56; 1-19; Rabin, BS. (1983). J. Nutr. 113(11); 2316 2322).

Forma física

Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.

Reconstitución

1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable