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Key Documents

MOD50

Sigma-Aldrich

Imprint® DNA Modification Kit

For bisulfite DNA conversion & purification

Synonym(s):

Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification

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About This Item

EC Number:
231-548-0
UNSPSC Code:
41116158
NACRES:
NA.54

Quality Level

200

usage

sufficient for 50 reactions

storage temp.

20-25°C

General description

The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

Application

Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.

Features and Benefits

  • Only 50 picograms of DNA or 20 cells are required
  • Procedure takes less than 2 hours
  • Greater than 99% conversion rate
  • Extremely low degradation
  • Option of convenient one-step protocol
  • Consistent and reproducible Bisulfite Modification
  • Can be used with genomic, endonuclease digested, and FFPE DNA

Storage and Stability

All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Kit Components Also Available Separately

Product No.
Description
SDS
  • T3566Clear-view™ Snap-Cap microtubes, size 1.5 mL, naturalSDS

Pictograms

CorrosionExclamation mark
GHS05,GHS07

Signal Word

Danger

Hazard Statements

H290,H302,H314,H412

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Supplementary Hazards

EUH031

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Elena Ivanova et al.
Clinical epigenetics, 12(1), 64-64 (2020-05-13)
Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation embryos in DNA methylation patterns and reprogramming. Here, we have extended
David E Condon et al.
BMC bioinformatics, 19(1), 31-31 (2018-02-07)
Identification of differentially methylated regions (DMRs) is the initial step towards the study of DNA methylation-mediated gene regulation. Previous approaches to call DMRs suffer from false prediction, use extreme resources, and/or require library installation and input conversion. We developed a
Nelly Olova et al.
Genome biology, 19(1), 33-33 (2018-03-17)
Whole-genome bisulfite sequencing (WGBS) is becoming an increasingly accessible technique, used widely for both fundamental and disease-oriented research. Library preparation methods benefit from a variety of available kits, polymerases and bisulfite conversion protocols. Although some steps in the procedure, such
Hwan Young Lee et al.
International journal of legal medicine, 126(1), 55-62 (2011-06-01)
DNA analysis of various body fluid stains at crime scenes facilitates the identification of individuals but does not currently determine the type and origin of the biological material. Recent advances in whole genome epigenetic analysis indicate that chromosome pieces called
Priyadarshini Kachroo et al.
Epigenomics, 10(2), 133-147 (2018-01-16)
To determine whether methylation differences between mostly fatal TCF3-HLF and curable TCF3-PBX1 pediatric acute lymphoblastic leukemia subtypes can be associated with differential gene expression and remission. Five (extremely rare) TCF3-HLF versus five (very similar) TCF3-PBX1 patients were sampled before and
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Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

DNA Methylation & Bisulfite Conversion

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DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

DNA Methylation & Bisulfite Conversion

Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.

Protocols

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Related Content

Imprint ® DNA Modification Kit (MOD50) Protocol

The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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