F6140

Anti-Fibronectin Antibody

mouse monoclonal, FN-3E2

• Synonym(s): Anti-CIG, Anti-ED-B, Anti-FINC, Anti-FN, Anti-FNZ, Anti-GFND, Anti-GFND2, Anti-LETS, Anti-MSF, Anti-SMDCF
• MDL number: MFCD00164562
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• product name: Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse, clone FN-3E2, ascites fluid
• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: FN-3E2, monoclonal
• mol wt: antigen 240 kDa
• contains: 15 mM sodium azide
• species reactivity: mouse, human, chicken
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect immunofluorescence: 1:400 using cultured chicken fibroblasts; radioimmunoassay: suitable; western blot: suitable
• isotype: IgM
• UniProt accession no.: P02751
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FN1(2335) ; mouse ... Fn1(14268)

Description

General description

Monoclonal Anti-Cellular Fibronectin (mouse IgM isotype) is derived from the hybridoma produced the fusion of mouse myeloma cells and splenocytes from immunized mice. Fibronectin is an extracellular matrix protein composed of two nearly identical disulfide-bound polypeptides with typical molecular weights of 220-240 kDa. Cellular fibronectin is structurally and antigenically similar to cold insoluble globulin from plasma and polyclonal antibodies to either form usually cross-react. The protein contains several functionally and structurally distinct domains, which may bind to cell surfaces, collagen, heparin, etc.

Specificity

The antibody labels cellular fibronectin in the pericellular matrix of chick embryo and mouse and human fibroblasts grown in tissue culture. It may be used for immunoaffinity purification of cellular fibronectin.

Immunogen

fibronectin antigen released in culture by a human breast cancer cell line

Application

Monoclonal Anti-Fibronectin, Cellular antibody produced in mouse has been used in:

• immunoblot
• immunofluorescent microscopy
• immunocytochemistry

Biochem/physiol Actions

Fibronectin is a soluble plasma protein that regulates tissue repair and thrombus formation. Insoluble fibronectin is expressed in basement membranes and connective tissue matrix and mainly modulates cell adhesion and migration. Alterations in fibronectin have been associated with microvascular thrombosis, disseminated intravascular coagulation, and atherosclerosis .

Fibronectin may enhance cell adhesion and spreading and affect the routes of cell migration both in vivo and in culture. It has been shown that upon malignant transformation many cells lose their surface bound fibronectin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable