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GE17-5072-01

Q Sepharose Xl

Cytiva 17-5072-01, pack of 300 mL

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

quaternary amine

description

Ion Exchanger Type (value)

packaging

pack of 300 mL

manufacturer/tradename

Cytiva 17-5072-01

matrix

6% cross-linked agarose with dextran surface extenders

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-14

working range

2-12

suitability

suitable for bioprocess medium

General description

Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.

With its high loading capacities and excellent physical and chemical stability, Q Sepharose XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.

As member of the BioProcess media range, Q Sepharose XL meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • High loading capacities give more productive capture from clarified feedstocks.
  • Strong anion exchanger designed for capture steps
  • High chemical stability for effective CIP/sanitization
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

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Protocols

This page covers how and when to use Sepharose XL media for purification of proteins.

This page discusses how to do a purification with SOURCE™ media from Cytiva.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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