Q1126
Q Sepharose™ Fast Flow
preswollen, 45-165 μm (wet), exclusion limit ~4,000,000 Da
Synonym(s):
Q Sepharose™
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About This Item
Recommended Products
form
preswollen
Quality Level
technique(s)
RNA extraction: suitable
particle size
45-165 μm (wet)
pore size
~4,000,000 Da exclusion limit
capacity
0.18-0.24 mmol Cl-/mL, packed gel
storage temp.
2-8°C
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General description
−Ο−CH2CHOHCH2OCH2CHOHCH2N+(CH3)3
Application
Q Sepharose™ is used in protein chromatography, ion exchange chromatography and anion exchange media. Q Sepharose™ has been used to study compounds of plant defense with applications for natural control of phytopathogenic fungi. Q Sepharose™has also been used to develop an efficient method for extracting high-quality mRNA from soil and to study the immunomodulatory proteins from garlic (Allium sativum).
Physical form
Preswollen in 20% ethanol
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
100.4 - 109.4 °F - closed cup
Flash Point(C)
38 - 43 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Here, we report an efficient method for extracting high-quality mRNA from soil. Key steps in the isolation of total RNA were low-pH extraction (pH 5.0) and Q-Sepharose chromatography. The removal efficiency of humic acids was 94 to 98% for all
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