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Key Documents

SRP2086

Sigma-Aldrich

p300 (C1135-2414) human

recombinant, expressed in insect cells, ≥70% (SDS-PAGE)

Synonym(s):

KAT3B, RP1-85F18.1, RSTS2, p300

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.26

biological source

human

recombinant

expressed in insect cells

Assay

≥70% (SDS-PAGE)

form

frozen liquid

mol wt

~145 kDa

packaging

pkg of 4 μg

concentration

350 μg/mL

color

clear colorless

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EP300(2033)

General description

E1A binding protein p300 (p300) has a central bromodomain and three cysteine- and histidine-rich regions. The carboxy-terminal region interacts with the adenovirus oncoprotein E1A. The gene encoding this protein is localized on human chromosome 22q13.

Biochem/physiol Actions

Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays. In addition to interacting with a variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as a tumor suppressor. The C-terminus of p300 contains a histone acetyltransferase (HAT) domain expanded from residues 1195 to 1673, an activation domain (residues 1763-1812) that interacts with many transcription factors and an SRC-1 domain further down stream of the activation domain. P300 is able to acetylate histones and many other transcription factors, including p53, E2F, TFIIE, and TFIIF etc. implicating diverse roles of p300 in gene regulation.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R W Stein et al.
Journal of virology, 64(9), 4421-4427 (1990-09-01)
Adenovirus E1A transforming function requires two distinct regions of the protein. Transforming activity is closely linked with the presence of a region designated conserved domain 2 and the ability of this region to bind the product of the cellular retinoblastoma
R Eckner et al.
Genes & development, 8(8), 869-884 (1994-04-15)
The growth-controlling functions of the adenovirus E1A oncoprotein depend on its ability ot interact with a set of cellular proteins. Among these are the retinoblastoma protein, p107, p130, and p300. We have isolated a cDNA encoding full-length human p300 and
Expanding the phenotypic spectrum in EP300-related Rubinstein-Taybi syndrome.
Solomon BD
American Journal of Medical Genetics. Part A, 167A(5), 1111-1116 (2015)
J C Chrivia et al.
Nature, 365(6449), 855-859 (1993-10-28)
Cyclic AMP-regulated gene expression frequently involves a DNA element known as the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. This modification
Molecular cloning and functional analysis of the adenovirus E1A-associated 300-kD protein (p300) reveals a protein with properties of a transcriptional adaptor.
Eckner R
Genes & Development, 8(8), 869-884 (1994)

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