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G0411

Sigma-Aldrich

Prionex® Highly purified Type A

aqueous solution

Synonym(s):

Gelatin, Prionex® Highly purified Type A

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About This Item

CAS Number:
9000-70-8
EC Number:
232-554-6
MDL number:
MFCD00081638
UNSPSC Code:
12352202
NACRES:
NA.61

biological source

Porcine bone

Quality Level

200

sterility

aseptically processed

form

aqueous solution

technique(s)

ELISA: suitable
microbiological culture: suitable
western blot: suitable

General description

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. The proteins are extracted by boiling skin, tendons, ligaments, bones, etc. in water. Type-A gelatin is derived from acid-cured (acid-hydrolyzed) tissue. Type-B gelatin is derived from lime-cured (base/alkaline-hydrolyzed) tissue. Gelatin is a biocompatible polymer.

Application

Prionex® Highly purified Type A has been used:
  • for coating cell culture to improve attachment of cells, and was added to polymerase chain reaction (PCR) to help stabilize Taq DNA
  • as a blocking reagent in Western blotting, enzyme linked immunosorbent assay (ELISA), and immunohistochemistry
  • as a component of media for species differentiation in bacteriology
  • as a delivery vehicle for the release of bioactive molecules and in the generation of scaffolds for engineering applications
  • in the murine lethality assay which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo

Legal Information

Prionex is a registered trademark of Pentapharm AG, Basel

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Encyclopedia of Materials: Science and Technology, 3495-3497 (2001)
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Encyclopedia of Physical Science and Technology, 91-132 (2001)
Amino Acid Composition of Gelatin from Ephinephelus sp.
Siregar GRM and Suprayitno E
IOSR Journal of Agriculture and veterinary Science, 12(4), 51-54 (2019)
Rosa Barreira da Silva et al.
Bio-protocol, 7(6), e2184-e2184 (2017-03-20)
Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is constitutively expressed in the extracellular space.
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