A9469

Monoclonal ANTI-FLAG^® M2-Alkaline Phosphatase antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Monoclonal ANTI-FLAG^® M2, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 41106514
• Conjugate: alkaline phosphatase conjugate
• Clone: M2, monoclonal
• Application: ELISA (i)
• Citations: 54

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: all
• concentration: ~1 mg/mL
• technique(s): indirect ELISA: 1:20,000
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... ALPL(249)

Description

General description

Monoclonal ANTI-FLAG^® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG^® fusion proteins and will recognize the FLAG^® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG^® peptides).

Application

Monoclonal ANTI-FLAG^® M2-Alkaline Phosphatase antibody produced in mouse has been used:

• in direct tissue blot immunoassay of sweet orange petioles samples[1]
• in screening internalization of delta opioid receptor
• for screening cell-free protein expression using ELISA

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable