A8592

ANTI-FLAG^® M2-Peroxidase (HRP) antibody, Mouse monoclonal

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

• Synonym(s): Monoclonal ANTI-FLAG^® M2, Anti-ddddk, Anti-dykddddk
• NACRES: NA.32
• UNSPSC Code: 41106514
• Conjugate: peroxidase conjugate
• Clone: M2, monoclonal
• Application: ELISA (i)
• Citations: 1904

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: M2, monoclonal
• form: buffered aqueous glycerol solution
• species reactivity: all
• concentration: ~1 mg/mL
• technique(s): indirect ELISA: 1:20,000
• isotype: IgG1
• immunogen sequence: DYKDDDDK
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... GPX1(2876)

Description

General description

The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.

Application

For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.

Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000

Learn more product details in our FLAG^® application portal.

Physical form

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Preparation Note

Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• pictograms: GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H314,H317,H410
• pcodes: P261 - P272 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
• Storage Class: 8A - Combustible corrosive hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable