Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Journal of agricultural and food chemistry (2004-08-19)

Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler

RÉSUMÉ

A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.

MATÉRIAUX

Référence du produit

Marque

Description du produit

57348-U

Supelco

Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex

57328-U

Supelco

Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, for use with manual holder

57298-U

Supelco

Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler

57299-U

Supelco

Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm

20358

Supelco

Tube ORBO^™ Carboxen-564, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

57329-U

Supelco

Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 24 ga, StableFlex, for use with autosampler

57318

Supelco

Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, for use with manual holder, needle size 24 ga

10478-U

Supelco

Adsorbant Carboxen^®, matrix Carboxen^® 1000, 60-80 mesh, bottle of 10 g

25467

Supelco

PLOT - Carboxen-1010, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

20355

Supelco

ORBO-78 400/200 MG PK/25, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25461

Supelco

PLOT - Carboxen-1006, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57295-U

Supelco

Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 23 ga, StableFlex, for use with autosampler

57319

Supelco

Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 24 ga, for use with autosampler

57335-U

Supelco

Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, StableFlex, for use with autosampler

W313203

Sigma-Aldrich

2-Isobutyl-3-méthoxypyrazine, ≥99%, FG

24246

Supelco

PLOT - Carboxen-1010, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

57334-U

Supelco

Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), d_f 85 μm(CAR/PDMS, needle size 24 ga, for use with manual holder, StableFlex fiber

57343-U

Supelco

Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with autosampler

47528-U

Supelco

2-Isobutyl-3-methoxypyrazine solution, certified reference material

297666

Sigma-Aldrich

2-Isobutyl-3-méthoxypyrazine, 99%

57344-U

Supelco

Unité de fibres SPME, carboxène/polydiméthylsiloxane (CAR/PDMS), d_f 75 μm(CAR/PDMS, needle size 23 ga, for use with manual holder

24241-U

Supelco

PLOT - Carboxen-1006, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

11072-U

Supelco

Adsorbant Carboxen^®, matrix Carboxen^® 572, 20-45 mesh, bottle of 10 g

28718-U

Supelco

Tenax^® TA / Carboxen^® 1018, glass TD tube, preconditioned, O.D. × L 1/4 in. × 3 1/2 in., Sealed with (Swagelok^® End-Fittings), pkg of 10 ea

54041-U

Supelco

Carboxen^® 1000 Reversible SPE Tube, 200 mg/mL, pk 30

57914-U

Supelco

Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), for use with autosampler, needle size 23 ga, metal alloy fiber, fiber L 2 cm

10477-U

Supelco

Adsorbant Carboxen^®, matrix Carboxen^® 1000, 40-60 mesh, bottle of 50 g

10471

Supelco

Adsorbant Carboxen^®, matrix Carboxen^® 1003, 40-60 mesh, bottle of 10 g

10269

Supelco

Adsorbant Carboxen^®, matrix Carboxen^® 569, 20-45 mesh, bottle of 10 g

23446-U

Supelco

Tube de sorbant ORBO^™-93, ORBO tube I.D. × L 6 mm × 95 mm, Bed A 180 mg, Bed B 90 mg, 60/80 mesh, pkg of 25 ea