ZRB69

Anti-Nestin Antibody, clone 8B8, ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonyme(s) : NES
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit (recombinant)
• Niveau de qualité: 200
• Produit recombinant: expressed in HEK 293 cells
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 8B8, monoclonal; recombinant monoclonal
• Description: 8B8 Clone
• Gamme de produits: ZooMAb^® learn more
• Forme: lyophilized
• Poids mol.: calculated mol wt 177.44 kDa; observed mol wt ~_220 kDa
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Caractéristiques du produit alternatif plus écologique: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Validation améliorée: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• Technique(s): immunocytochemistry: suitable using 1:100; immunohistochemistry: suitable using 1:100; western blot: suitable using 1:1,000
• Isotype: IgG
• Numéro d'accès UniProt: P48681
• Autre catégorie plus écologique: Aligned,
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... NES(10763)

Description

Description générale

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Spécificité

Clone 8B8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Nestin. It targets an epitope with in 150 amino acids from the C-terminal region.

Immunogène

GST/His-tagged recombinant fragment corresponding to 150 amino acids from the C-terminal region of human Nestin.

Application

Anti-Nestin, clone 8B8, ZooMAb is a recombinant Rabbit Monoclonal antibody that detects Nestin and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nestin in SH-SY5Y cells.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Nestin in lysate from Human H9 Stem Cells.

Description de la cible

Nestin (UniProt: P48681) is encoded by the NES (also known as Nbla00170) gene (Gene ID: 10763) in human. Nestin is a class VI intermediate filament protein that is required for survival, renewal, and mitogen-stimulated proliferation of neural progenitor cells. Nestin expression is up-regulated in most mitotic cells and is downregulated upon differentiation. Upon terminal neural differentiation Nestin levels are shown to be down-regulated and replaced by neurofilaments. Nestin is also shown to be expressed in kidney in a developmentally regulated pattern. Its expression is also associated with tubulointerstitial injury and can serve as a marker for peritubular endothelial cell injury and tubulointerstitial fibrosis. Nestin is reported to be constitutively phosphorylated and its phosphorylation state increases during mitosis when the cytoplasmic intermediate filament network is reorganized. Nestin has a short N-terminus and an unusually long C-terminus region that results in complex binding to an array of structural proteins. The short N-terminus leads to preferential intermediate filaments formation with heterodimers and heterotetramers and the long C-terminal portion functions as links or bridges between intermediate filaments and microtubules. Nestin can exist in homodimeric or homotetrameric form, however in mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, it can form heterodimers, which can assemble to form intermediate filaments. Nestin is shown to promote the disassembly of phosphorylated vimentin intermediate filaments during mitosis and play a role in the trafficking and distribution of intermediate filament proteins to daughter cells during progenitor cell division. (Ref.: Xie, L., et al. (2015). Stem Cells Int. 2015:762098; Tomioka, M., et al. (2010). 15(5); 568-574).

Forme physique

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informations légales

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 2