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Key Documents

Y1003

Sigma-Aldrich

2x YT medium

Liquid microbial growth medium

Synonyme(s) :

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium

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About This Item

Code UNSPSC :
41106225
Nomenclature NACRES :
NA.85

Qualité

for molecular biology

Niveau de qualité

300

Stérilité

sterile; 0.2 μm filtered

Forme

liquid

Technique(s)

microbiological culture: suitable

pH

6.5-7.5

Application(s)

microbiology

Température de stockage

room temp

Adéquation

nonselective for Escherichia coli
nonselective for coliforms

Catégories apparentées

Description générale

2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.

Caractéristiques et avantages

2xYT liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Composants

16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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