XNABR
REDExtract-N-Amp™ Blood PCR Kit
sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonyme(s) :
Blood direct PCR kit, Blood direct PCR kit with loading dye
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About This Item
Code UNSPSC :
41106303
Nomenclature NACRES :
NA.55
Produits recommandés
Utilisation
sufficient for 1000 amplifications
sufficient for 1000 extractions
sufficient for 1000 reactions
Niveau de qualité
Caractéristiques
dNTPs included
hotstart
Technique(s)
PCR: suitable
Couleur
red
Conditions d'expédition
wet ice
Température de stockage
−20°C
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Description générale
The REDExtract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Application
REDExtract-N-Amp™ Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, parasites and endocervical swab eluates of mice. It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.
Caractéristiques et avantages
- Efficient 8 minute prep allows greater speed and throughput
- No need for organic extraction, centrifugation or alcohol precipitation
- Hot start antibody for highly specific PCR amplification of genomic DNA
- Can be used with whole blood or blood cards
- Extract stable at 4 °C for at least 6 months
- Compatible with any format (single tube, 96-well, etc.)
- Simple, 3 step procedure with no special equipment required
- REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysis
- PCR products are suitable for direct cloning and sequencing
Principe
Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.
Autres remarques
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Informations légales
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Code de la classe de stockage
8A - Combustible corrosive hazardous materials
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Sofia Lejon Crottet et al.
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis, 50(2), 169-174 (2014-04-01)
Starting in 2013, blood donors must be tested at least using: (1) one monoclonal anti-D and one anti-CDE (alternatively full RhCcEe phenotyping), and (2) all RhD negative donors must be tested for RHD exons 5 and 10 plus one further
Olivier A E Sparagano et al.
Annals of the New York Academy of Sciences, 1081, 505-508 (2006-12-01)
Our study is based on the collection of blood and ticks from sheep in Iran and Italy. Polymerase chain reaction (PCR) testing was performed to target the 18S rRNA gene and RLB was performed using previously published probes. In Italy
Anna K Nowińska et al.
Molecular vision, 17, 2333-2342 (2011-09-17)
To analyze genotype-phenotype correlation in patients originating from Polish population with the transforming growth factor beta induced (TGFBI) corneal dystrophies. Sixty affected and 31 unaffected individuals from 15 unrelated Polish families were included in the study. The clinical diagnosis was
Dawn M Reeves et al.
Antimicrobial agents and chemotherapy, 51(7), 2646-2648 (2007-05-16)
Mice treated with antibiotics early or late after active infection had resolved were examined for chlamydial DNA in endocervical swabs. The early eradication of infection limited oviduct pathology, despite the continued detection of chlamydial DNA by nested PCR. Late antibiotic
Timothy S Fenske et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(42), 15184-15189 (2004-10-13)
The t(8;21)(q22;q22) translocation, present in 10-15% of acute myeloid leukemia (AML) cases, generates the AML1/ETO fusion protein. To study the role of AML1/ETO in the pathogenesis of AML, we used the Ly6A locus that encodes the well characterized hematopoietic stem
Articles
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
Extract-N-Amp simplifies DNA extraction and amplification, enabling PCR directly from FTA blood cards, reducing variability.
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