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Key Documents

N9784

Sigma-Aldrich

Neutralization Solution for Blood

sufficient for 100 reactions, sufficient for 1000 reactions, for molecular biology

Synonyme(s) :

DNA neutralization solution, Neutralization buffer DNA extraction

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Niveau de qualité

Forme

liquid

Utilisation

sufficient for 100 reactions
sufficient for 1000 reactions

Température de stockage

room temp

Description générale

Neutralization Solution for Blood is used along with lysis solution for blood (L3289) for the rapid extraction and neutralization of human genomic DNA from whole blood, whole blood dried on a blood card, and cultured mammalian cells.

This solution is a component of the Extract-N-Amp and the REDExtract-N-Amp Blood PCR Kits

Application

Neutralization Solution for blood has been used to neutralize extracted DNA prior to polymerase chain reaction (PCR) amplification.

Informations légales

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Martina Veith et al.
International journal of chronic obstructive pulmonary disease, 14, 2535-2542 (2019-12-11)
Alpha-1-antitrypsin deficiency (AATD) is a rare hereditary condition resulting from the mutations in the SERPINA1 (serine protease inhibitor) gene and is characterized by low circulating levels of the alpha-1 antitrypsin (AAT) protein. The traditional algorithm for laboratory testing of AATD
Leandro S Thiago et al.
Haematologica, 99(1), 155-162 (2013-07-23)
The B-cell compartment in which multiple myeloma stem cells reside remains unclear. We investigated the potential presence of mature, surface-membrane immunoglobulin-positive B lymphocytes clonally related to the tumor bone marrow plasma cells among different subsets of peripheral blood B cells
G Henderson et al.
Archives of disease in childhood. Fetal and neonatal edition, 94(2), F124-F128 (2007-09-05)
The inflammatory cytokine cascade is implicated in the pathogenesis of necrotising enterocolitis (NEC). Genetic association studies of cytokine polymorphisms may help to detect molecular mechanisms that are causally related to the disease process. To examine associations between the common genetic
Huiling Wu et al.
The Journal of clinical investigation, 117(10), 2847-2859 (2007-09-15)
Ischemia/reperfusion injury (IRI) may activate innate immunity through the engagement of TLRs by endogenous ligands. TLR4 expressed within the kidney is a potential mediator of innate activation and inflammation. Using a mouse model of kidney IRI, we demonstrated a significant
Aaron Zhong et al.
STAR protocols, 1(2) (2020-10-20)
Reporter cell lines based on human pluripotent stem cells (hPSCs) are highly desirable for studying differentiation, lineage tracing, and target cell selection. However, several technical bottlenecks, such as DNA transduction, low homology recombination rate (HDR), and single-cell cloning, have made

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