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U0508

Sigma-Aldrich

Monoclonal Anti-Ubiquitin antibody produced in mouse

clone 6C1, ascites fluid

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About This Item

Numéro MDL:
MFCD03792436
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

ascites fluid

Type de produit anticorps

primary antibodies

Clone

6C1, monoclonal

Contient

15 mM sodium azide

Espèces réactives

human, rat, mouse, bovine

Technique(s)

indirect ELISA: suitable
microarray: suitable
western blot: 1:1,000 using whole cell lysate of human promyelocitic HL60 cells

Isotype

IgG2a

Numéro d'accès UniProt

P0CG47

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... UBB(7314) , UBC(7316)
mouse ... Ubb(22187) , Ubc(22190)
rat ... Ubb(192255) , Ubc(50522)

Description générale

Ubiquitin, an 8.5kD (approx.) 76 amino acid containing protein, is highly conserved and found in both prokaryotes and eukaryotes. It facilitates protein degradation in ubiquitin/proteasome pathway and also has pivotal role in intracellular regulation. Monoclonal anti-ubiquitin antibody can be used in immunoblotting and ELISA to localize and detect ubiquitin. Mouse anti-ubiquitin antibody reacts specifically with ubiquitin in mouse, human, bovine and rat.

Immunogène

purified bovine ubiquitin conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Monoclonal anti-ubiquitin antibody can be used in western blot to visualize and evaluate ubiquitylated proteins.It can also be used in microarray and immunoblotting.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Yuga Takahashi et al.
FEBS letters, 585(12), 1741-1747 (2011-05-17)
Nerve growth factor (NGF) binding to its receptor TrkA, which belongs to the family of receptor tyrosine kinases (RTKs), is known to induce its internalization, endosomal trafficking and subsequent lysosomal degradation. The Cbl family of ubiquitin ligases plays a major
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The full enzymatic activity of the cullin-RING ubiquitin ligases (CRLs) requires a ubiquitin-like protein (that is, Nedd8) modification. By deamidating Gln40 of Nedd8 to glutamate (Q40E), the bacterial cycle-inhibiting factor (Cif) family is able to inhibit CRL E3 activities, thereby
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