T5816
Tricine
BioPerformance Certified, suitable for cell culture, ≥99% (titration)
Synonyme(s) :
N-[Tris(hydroxyméthyl)méthyl]glycine
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About This Item
Formule linéaire :
(HOCH2)3CNHCH2CO2H
Numéro CAS:
Poids moléculaire :
179.17
Numéro Beilstein :
1937804
Numéro CE :
Numéro MDL:
Code UNSPSC :
12161700
ID de substance PubChem :
Nomenclature NACRES :
NA.25
Produits recommandés
Qualité
BioPerformance Certified
Pureté
≥99% (titration)
Forme
crystalline powder
Conditions de stockage
dry at room temperature
Technique(s)
cell culture | mammalian: suitable
Impuretés
endotoxin and total aerobic microbial count, tested
Couleur
white
Plage de pH utile
7.4-8.8
pKa (25 °C)
8.1
Pf
187 °C
Traces de cations
heavy metals (as Pb): ≤5 ppm
Absorption
≤0.02 at 290 at 20%
Application(s)
diagnostic assay manufacturing
general analytical
life science and biopharma
sample preparation
Chaîne SMILES
OCC(CO)(CO)NCC(O)=O
InChI
1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
Clé InChI
SEQKRHFRPICQDD-UHFFFAOYSA-N
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Description générale
Tricine is a zwitterionic biological buffer commonly used in enzyme assays, electrophoresis, and cell culture research. It has a higher negative charge compared to glycine, which enables it to migrate more rapidly. Tricine′s high ionic strength has the ability to cause more ion movement and less protein movement, resulting in the separation of low molecular weight proteins in lower percent acrylamide gels. It has been observed that Tricine is efficient in the separation of proteins ranging from 1 to 100 kDa through electrophoresis. According to a study, the 25 mmol/L Tricine buffer proved to be the most effective among ten other buffers tested for ATP assays using firefly luciferase. Further, Tricine is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography.
Application
Tricine serves as a versatile buffering agent suitable for separating low molecular weight proteins in lower percent acrylamide gels, has demonstrated efficacy in ATP assays employing firefly luciferase, and has shown potential in scavenging hydroxyl radicals during the investigation of radiation-induced membrane damage. Additionally, it has played a role in isolating and purifying ribosomes engaged in the translation of specific mRNA and containing specific peptidyl-tRNA molecules. Tricine has also been employed in the synthesis of RNA oligonucleotides and the fabrication of high-density DNA microarrays, enabling the exploration of mechanisms underlying nucleic acid·ligand interactions and library preparation through photolithography.
Constituant de tampons destinés à la séparation de peptides de faible poids moléculaire.
Caractéristiques et avantages
- Suitable as a Buffer component, for Electrophoresis and Protein separation
- Effective Buffering from pH 7.4-8.8 (25 °C) with a pKa of 8.1 (25 °C)
- Tested for Endotoxins and Total Aerobic Microbial Count
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Autres remarques
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Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Certificats d'analyse (COA)
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Isolation of translating ribosomes containing peptidyl-tRNAs for functional and structural analyses.
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Totally porous lipid-based liquid crystalline nanoparticles were used as pseudostationary phase for capillary electroseparation with LIF detection of proteins at physiological conditions using unmodified cyclic olefin copolymer capillaries (Topas, 6.7 cm effective length). In the absence of nanoparticles, i.e. in
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