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T5694

Sigma-Aldrich

TRAIL human

recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder

Synonyme(s) :

Apo2 Ligand, TL2

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About This Item

Numéro CAS:
2244730-93-4
Numéro MDL:
MFCD01867158
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

human

Niveau de qualité

200

Produit recombinant

expressed in NSO cells

Description

N-terminal 6X histidine-tagged extracellular domain

Pureté

>97% (SDS-PAGE)

Forme

lyophilized powder

Poids mol.

24 kDa by SDS-PAGE
predicted mol wt ~21 kDa

Technique(s)

cell culture | mammalian: suitable

Impuretés

endotoxin, tested

Numéro d'accès UniProt

P50591

Température de stockage

−20°C

Informations sur le gène

human ... TNFSF10(8743)

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Application

TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line.

Actions biochimiques/physiologiques

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells.
Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

Forme physique

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Remarque sur l'analyse

Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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J Sträter et al.
Annals of the New York Academy of Sciences, 915, 162-170 (2001-02-24)
The tumor necrosis factor receptor (TNFR) family is a still-growing group of homologous transmembrane proteins, some of which bear an intracellular "death domain" and are able to directly mediate apoptosis. Apoptosis is induced upon trimerization of the receptors by their
Bich Hang Do et al.
Journal of microbiology and biotechnology, 27(12), 2156-2164 (2017-10-17)
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered as an antitumor agent owing to its ability to induce apoptosis of cancer cells without imparting toxicity toward most normal cells. TRAIL is produced in poor yield because of its insoluble expression
X D Zhang et al.
FEBS letters, 482(3), 193-199 (2000-10-12)
Resistance of normal cells to tumour necrosis factor related apoptosis inducing ligand (TRAIL) induced apoptosis is believed to be mediated by expression of two decoy receptors. Here we show that the expression and localisation of TRAIL receptors (TRAIL-Rs) vary between
W S Simonet et al.
Cell, 89(2), 309-319 (1997-04-18)
A novel secreted glycoprotein that regulates bone resorption has been identified. The protein, termed Osteoprotegerin (OPG), is a novel member of the TNF receptor superfamily. In vivo, hepatic expression of OPG in transgenic mice results in a profound yet nonlethal
Ling Xu et al.
Oncology reports, 32(1), 318-324 (2014-05-21)
Gastric cancer cells are insensitive to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), and we recently showed that lipid raft-regulated epidermal growth factor receptor (EGFR) activation antagonized TRAIL-induced apoptosis. However, it is not clear whether caveolin-1, an essential structural constituent of
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