T2551
Monoclonal Anti-Tenascin antibody produced in mouse
clone BC-24, ascites fluid
Synonyme(s) :
Anti-Tenascin-N
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About This Item
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
ascites fluid
Type de produit anticorps
primary antibodies
Clone
BC-24, monoclonal
Contient
15 mM sodium azide
Espèces réactives
human
Technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:4,000 using protease-digested sections of adult human tissue.
immunohistochemistry (frozen sections): suitable
indirect ELISA: suitable
microarray: suitable
radioimmunoassay: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
−20°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TNN(63923)
Description générale
Monoclonal Anti-Human Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.
Tenascin (TN) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. Human TN has 3 subunits of 190, 200 and 220 kD. TN has been independently discovered in a variety of species and tissue types, often in the basement membrane or intercellular spaces. The expression of TN is associated with development and growth, both normal and pathological, with restricted distribution in normal adult tissue. It is synthesized by fibroblasts, chondroblasts, osteocytes, smooth muscle cells and glial cells. Human and chicken TN contain an RGD sequence which may function in cell adhesion and it seems likely that TN mediates cell attachment through an RGD-dependent integrin receptor. Mouse TN does not contain an RGD sequence in the third type III repeat implicated in cell attachment, or in any other positions.
Spécificité
The antibody recognizes an epitope within the N-terminal EGF-like (EGF-L) sequence of the molecule. It recognizes all human tenascin isoforms.
Immunogène
human tenascin.
Application
Monoclonal Anti-Tenascin antibody produced in mouse has been used for immunohistochemistry.
Mouse monoclonal clone BC-24 anti-Tenascin antibody is an essential tool for the localization, identification and studies on the role of tenascin in epithelial-mesenchymal and neuronal-glial interactions.
Actions biochimiques/physiologiques
Tenascin (TN) is up-regulated by epithelial transforming growth factor β (TGF-β) in mesenchyme. TN in the stroma of cancerous tissue contributes to tumorigenesis and progression of metastasis. High molecular weight TN isoform, a stromal marker of breast cancer, over-expresses implicating cell proliferation and invasion. TN has a potential role in tissue restoration. Intracytoplasmic TN immunoreactivity has been detected in malignant melanomas and in lung carcinomas. TN expression, when reacted with monoclonal antibody, determines its localization in tumor cells and its association with epithelial-mesenchymal and neuronal-glial cell interactions.
Forme physique
This product is supplied as ascites fluid containing 15 mM sodium azide
Stockage et stabilité
For continuous use, store at 2-8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Tenascin-C in primary malignant melanoma of the skin
Ilmonen S, et al.
Histopathology, 45(4), 405-411 (2004)
Expression of annexin II and stromal tenascin C promotes epithelial to mesenchymal transition and correlates with distant metastasis in pancreatic cancer
Yoneura N, et al.
International Journal of Molecular Medicine, 42(2), 821-830 (2018)
Chun-Chao Wang et al.
Nature cell biology, 16(4), 345-356 (2014-03-25)
Basal-like breast carcinoma is characterized by poor prognosis and high intratumour heterogeneity. In an immortalized basal-like breast epithelial cell line, we identified two anticorrelated gene-expression programs that arise among single extracellular matrix (ECM)-attached cells during organotypic three-dimensional culture. The first
G I Mason et al.
Annals of the rheumatic diseases, 59(3), 183-189 (2000-03-04)
To determine whether there was altered elaboration of non-collagenous extracellular matrix proteins or expression of TGFbeta isoforms and their receptors in salivary glands of patients with systemic sclerosis (SSc) and Raynaud's phenomenon (RP). Because of the possible role of mast
Involvement of large tenascin-C splice variants in breast cancer progression
Tsunoda T, et al.
The American Journal of Pathology, 162(6), 1857-1867 (2003)
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