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T2391

Sigma-Aldrich

Triosephosphate Isomerase from rabbit muscle

Type III-S, ammonium sulfate suspension, ≥4,000 units/mg protein

Synonyme(s) :

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

Numéro CAS:
9023-78-3
Numéro de classification (Commission des enzymes):
5.3.1.1 (BRENDA, IUBMB)
Numéro MDL:
MFCD00082080
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Source biologique

rabbit muscle

Niveau de qualité

200

Type

Type III-S

Forme

ammonium sulfate suspension

Activité spécifique

≥4,000 units/mg protein

Poids mol.

calculated mol wt 53.2 kDa

Activité étrangère

3-phosphoglyceric phosphokinase ≤0.01%
glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
pyruvate kinase, lactic dehydrogenase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase and aldolase ≤0.001%

Température de stockage

2-8°C

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Catégories apparentées

Application

Triosephosphate isomerase has been used in a study to assess molecular characterizations of Cryptosporidium, Giardia, and Enterocytozoon. Triosephosphate isomerase has also been used in a study to investigate apoptosis of hepatocellular carcinoma cell lines.

Définition de l'unité

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Forme physique

Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0

Remarque sur l'analyse

Protein determined by biuret

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Yi-Lin Sophia Chen et al.
Peptides, 36(2), 257-265 (2012-06-05)
Due to its malignancy, the development of effective therapeutic strategies for hepatocellular carcinoma (HCC) is of urgent needs. Natural antimicrobial peptides (AMPs), also known as host defense peptides (HDPs), not only act as direct antimicrobial agents, but also represent important
Beatty V Maikai et al.
Experimental parasitology, 131(4), 452-456 (2012-06-06)
The use of molecular diagnostic tools in epidemiological investigations of Cryptosporidium, Giardia, and Enterocytozoon has provided new insights into their diversity and transmission pathways. In this study, 157 stool specimens from 2-month to 70-year-old patients were collected, a polymerase chain
Susan K Boehlein et al.
The Plant journal : for cell and molecular biology, 99(1), 23-40 (2019-02-13)
Cereal yields decrease when grain fill proceeds under conditions of prolonged, moderately elevated temperatures. Endosperm-endogenous processes alter both rate and duration of dry weight gain, but underlying mechanisms remain unclear. Heat effects could be mediated by either abnormal, premature cessation
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 17(7), 576-584 (2016-01-12)
Nonribosomal peptide synthetases (NRPSs) produce many important and structurally complex natural products. Because of their architectures, reprogramming NRPSs has long been attempted to access new bioactive compounds. However, detailed characterization of NRPS catalysis and substrate selectivity by adenylation (A) domains
Rongchang Yang et al.
Experimental parasitology, 137, 46-52 (2013-12-21)
A novel quantitative PCR (qPCR) for Giardia at the glutamate dehydrogenase (gdh) locus was developed and validated. The qPCR was used to screen a total of 3412 lamb faecal samples collected from approximately 1189 lambs at three sampling periods (weaning
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Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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