SRP6053

ProBDNF human

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

• Synonyme(s) : Abrineurin, BDNF, Brain-derived neurotrophic factor
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥95% (HPLC); ≥95% (SDS-PAGE)
• Forme: lyophilized
• Poids mol.: ~52 kDa
• Conditionnement: pkg of 10 μg
• Numéro d'accès NCBI: P23560
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... BDNF(627)

Description

Description générale

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors. The precursor form of brain-derived neurotrophic factor (pro-BDNF) undergoes post-translational cleavage to form mature BDNF (mBDNF). The gene encoding this protein is localized on human chromosome 11p14.1.

Actions biochimiques/physiologiques

Precursor form of brain-derived neurotrophic factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) (2) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the tropomyosin-related kinase B (TrkB) kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.

Notes préparatoires

Centrifuge the vial prior to opening.

Reconstitution

Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.

Autres remarques

Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable