SRP3200
GDNF from mouse
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonyme(s) :
ATF-1, Glial-Derived Neurotrophic Factor
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32
Produits recommandés
Source biologique
mouse
Produit recombinant
expressed in E. coli
Pureté
≥98% (HPLC)
≥98% (SDS-PAGE)
Forme
lyophilized
Puissance
≤0.2 ng/mL
Poids mol.
15.1 kDa
Conditionnement
pkg of 10 μg
Technique(s)
cell culture | mammalian: suitable
Impuretés
<0.1 EU/μg endotoxin, tested
Couleur
white to off-white
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Température de stockage
−20°C
Informations sur le gène
mouse ... GDNF(14573)
Description générale
GDNF (glial cell line derived neurotrophic factor) is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. The functional murine GDNF ligand is a disulfide-linked homodimer, of two 15.1kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Application
GDNF from mouse has been used for stimulating primary murine cultures of dorsal root ganglion (DRG) neurons.
GDNF from mouse has been used to study the role of NEDL2 (Nedd4-related E3 ubiquitin ligase-2) in GDNF/Ret signaling.
Actions biochimiques/physiologiques
GDNF (glial cell line derived neurotrophic factor) signals through a multicomponent receptor system, composed of a RET (receptor tyrosine kinase) and one of the four GFR-α (α1-α4) receptors (GDNF family receptors). GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson′s disease monkey model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. It might also be involved in the treatment of non-alcoholic fatty liver disease. GDNF is needed for proper spermatogonial stem cell survival and proliferation.
Séquence
MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI
Forme physique
Lyophilized from 10 mM Sodium Citrate.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Les clients ont également consulté
Glial cell line-derived neurotrophic factor protects against high-fat diet-induced hepatic steatosis by suppressing hepatic PPAR-? expression.
Mwangi SM, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 310, G103-G116 (2016)
GDNF - a stranger in the TGF-beta superfamily?
Saarma M
European Journal of Biochemistry, 267, 6968-6971 (2000)
A rapid induction mechanism for Lin28a in trophic responses
Amen Am, et al.
Molecular Cell, 65(3), 490-503 (2017)
M Trupp et al.
Nature, 381(6585), 785-789 (1996-06-27)
Glial-cell-line-derived neutrophic factor (GDNF) promotes the survival and phenotype of central dopaminergic noradrenergic and motor neurons, as well as various subpopulations of peripheral sensory and sympathetic neurons. GDNF is structurally related to members of the transforming growth factor (TGF)-beta superfamily
NEDL2 is an essential regulator of enteric neural development and GDNF/Ret signaling.
Wei R, et al.
Cellular Signalling, 27, 578-586 (2015)
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