SAE0045

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

• Synonyme(s) : Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: human (human Rhinovirus Type 14)
• Niveau de qualité: 200
• Pureté: ≥90% (SDS-PAGE)
• Forme: aqueous solution
• Activité spécifique: ≥5000 U/mg
• Poids mol.: 21 kDa
• Concentration: 0.8-1.2 mg/mL
• Technique(s): protein purification: suitable
• Adéquation: suitable for protein modification
• Application(s): life science and biopharma
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Application

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Définition de l'unité

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Forme physique

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2