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SAB4200485

Sigma-Aldrich

Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonyme(s) :

Anti-H3.4, Anti-H3/g, Anti-H3FT, Anti-H3K27ac, Anti-H3t, Anti-HIST3H3, Anti-Hist1h2ai, Anti-Hist1h2ail, Anti-Hist1h3a, Anti-Hist1h3g, Anti-Hist1h3h, Anti-Hist1h3i

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

200

Conjugué

unconjugated

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~15 kDa

Espèces réactives

human, rat, mouse

Concentration

~1.0 mg/mL

Technique(s)

immunohistochemistry: 10-20 μg/mL
indirect immunofluorescence: 2.5-5.0 μg/mL using HeLa cells
western blot: 1-2 μg/mL using extracts of NIH3T3 cells

Numéro d'accès UniProt

Q16695

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

acetylation (Lys27)

Informations sur le gène

human ... HIST3H3(8290)

Description générale

The HIST3H3 gene is mapped on the human chromosome at 1q42.13. Histones are proteins that form the nucleosome along with DNA. A nucleosome consists of core histone octamers made of two heterodimers of H2A and H2B along with two heterodimers of H3 and H4. This octamer is bound by the DNA and H1 protein.

Spécificité

Anti-acetyl-Histone H3 (Ac-Lys27) specifically recognizes human and mouse (Ac-Lys27) Histone H3 (H3K27Ac).

Immunogène

synthetic peptide containing acetylated Lys27 of human histone H3, conjugated to KLH. The corresponding sequence is identical in many species including rat and mouse histone H3

Application

Anti-acetyl-Histone H3 (Ac-Lys27) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Actions biochimiques/physiologiques

Histones are subjected to extensive covalent modifications, including phosphorylation, methylation, acetylation and ubiquitination. Active chromatin is highly correlated with the hyperacetylation of histone H3 at the N-terminal tail. Histone H3 can be reversibly acetylated at Lys residues 9, 14, 18, 23, 27, 36 and 56 by a family of enzymes known as histone acetyltransferases (HATs). Histone H3 can be modified at Lys27 either by methylation or acetylation. Histones are thought to play an important role in development and cancer.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Minghua Li et al.
Cell biology international, 45(6), 1278-1287 (2021-02-10)
One hallmark of renal cell carcinoma (RCC) is metabolic reprogramming, which involves elevation of glycolysis and upregulation of lipid metabolism. However, the mechanism of metabolic reprogramming is incompletely understood. Monocarboxylate transporter 1 (MCT1) promotes transport for lactate and pyruvate, which
Emanuely Silva Chrun et al.
Pathology, research and practice, 213(11), 1329-1339 (2017-09-09)
Among the epigenetic changes, histone acetylation has been recognized as a fundamental process that strongly affects gene expression regulation. Disrupt of this phenomenon has been linked to carcinogenesis. In this review, we analysed studies reporting the process of histone modification
Thomas H A Ederveen et al.
Biochimica et biophysica acta, 1809(10), 577-586 (2011-07-26)
Histones are highly basic, relatively small proteins that complex with DNA to form higher order structures that underlie chromosome topology. Of the four core histones H2A, H2B, H3 and H4, it is H3 that is most heavily modified at the

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