Accéder au contenu
Merck
Toutes les photos(2)

Principaux documents

S6147

Sigma-Aldrich

Sephadex® G-100

BioReagent, for molecular biology, DNA grade, medium

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.52

Le tarif et la disponibilité ne sont pas disponibles actuellement.

Qualité

DNA grade
for molecular biology

Niveau de qualité

Gamme de produits

BioReagent

Forme

beads

Technique(s)

buffer exchange: suitable

Groupe de la matrice active

phase

Gonflement

1 g swells to 9-11 mL

Diamètre des billes

40-120 μm

Dimension de pores

exclusion limit (25 bp dsDNA; (rA)27)

pH

2-10

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 4

Cet article
SML0248SML1926SML1886
ZCZ011 ≥98% (HPLC)

SML2710

ZCZ011

Zolmitriptan ≥98% (HPLC)

SML0248

Zolmitriptan

GAT211 ≥98% (HPLC)

SML1926

GAT211

VPC-13566 ≥98% (HPLC)

SML1886

VPC-13566

form

powder

form

powder

form

powder

form

powder

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

DMSO: 2 mg/mL, clear

solubility

DMSO: ≥5 mg/mL

solubility

DMSO: 10 mg/mL, clear

solubility

DMSO: 20 mg/mL, clear

color

white to beige

color

white to beige

color

white to beige

color

white to beige

Description générale

Sephadex® G-100, Medium is an often-cited gel filtration medium for desalting and buffer exchange of very large molecules. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-100 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify the enzyme polyphenoloxidases isolated from banana by gel filteration.[1]

Caractéristiques et avantages

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Informations légales

Sephadex is a registered trademark of Cytiva

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


  • Certificats d'analyse (COA)

    Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

    Déjà en possession de ce produit ?

    Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

    Consulter la Bibliothèque de documents

    Les clients ont également consulté

    Isolation, purification and physicochemical characterization of polyphenoloxidases (PPO) from a dwarf variety of banana (Musa cavendishii, L)
    Galeazzi MAM et al
    Journal of Food Science, 46, 150-155 (1981)
    Evan S Leibner et al.
    Acta biomaterialia, 5(5), 1389-1398 (2009-01-13)
    Analytical protocol greatly influences the measurement of human serum albumin (HSA) adsorption to commercial expanded polytetrafluororethylene (ePTFE) exhibiting superhydrophobic wetting properties. Degassing of buffer solutions and evacuation of ePTFE adsorbent to remove trapped air immediately prior to contact with protein
    S Kaushik et al.
    Methods in molecular biology (Clifton, N.J.), 445, 227-244 (2008-04-22)
    Chaperone-mediated autophagy (CMA) is the only type of autophagy in mammalian cells able to selectively degrade cytosolic proteins in lysosomes. CMA is maximally activated in response to stressors such as prolonged starvation, exposure to toxic compounds, or oxidative stress. We
    Sanjiv K Mishra et al.
    Protein expression and purification, 80(2), 234-238 (2011-07-13)
    C-phycoerythrin was isolated and purified from marine Pseudanabaena sp. using two step chromatographic methods. Phycobiliproteins in the marine Pseudanabaena was extracted in 100 mM phosphate buffer (pH 7.2) and precipitated by salting out. The precipitated C-phycoerythrin was purified by gel
    Fernanda O Lemos et al.
    European journal of dermatology : EJD, 21(5), 722-730 (2011-07-09)
    Previous studies demonstrated that proteinases from latex of C. candamarcensis act as mitogens on fibroblast and epithelial cells and a subsequent report showed their protective, angiogenic and wound healing effects on gastric ulcers. In this study, we present evidence of

    Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

    Contacter notre Service technique