Accéder au contenu
Merck
Toutes les photos(2)

Key Documents

View All Documentation

S6022

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, medium

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(2)

About This Item

Numéro CAS:
9004-54-0
Numéro MDL:
MFCD00132771
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.52

Qualité

DNA grade
for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

beads

Gonflement

1 g swells to 9-11 mL

Diamètre des billes

50-150 μm

Dimension de pores

exclusion limit (20 bp dsDNA; (rA)20)

pH

2-10

Activité étrangère

DNase, RNase, none detected

Température de stockage

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

Clé InChI

FZWBNHMXJMCXLU-UHFFFAOYSA-N

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Description générale

Sephadex® G-50, Medium is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Medium” indicates a large particle size for use in large-scale group separations where high flow rates and low operating pressures are required.

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing reactions.
Sephadex® G-50 has been used to purify the DNA products in cycle sequencing reactions.

Caractéristiques et avantages

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Informations légales

Sephadex is a registered trademark of Cytiva

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Les clients ont également consulté

Slide 1 of 5

1 of 5

Sephadex® G-100 BioReagent, for molecular biology, DNA grade, medium

Sigma-Aldrich

S6147

Sephadex® G-100

Sephadex® G-50 Superfine, Cytiva, pack of 100 g

GE17-0041-01

Sephadex® G-50

Sephadex® G-50 Fine, Cytiva, pack of 500 g

GE17-0042-02

Sephadex® G-50

Sephadex® G-50 DNA grade, Cytiva, pack of 25 g

GE17-0573-01

Sephadex® G-50

Sephadex® G-25 Coarse

Sigma-Aldrich

G25300

Sephadex® G-25

The history of a Nearctic colonization: molecular phylogenetics and biogeography of the Nearctic toads (Bufo)
Pauly GB, et al.
Evolution, 58(11), 2517-2535 (2004)
Alisha K Holloway et al.
The American naturalist, 167(4), E88-101 (2006-05-04)
Polyploidization is one of the few mechanisms that can produce instantaneous speciation. Multiple origins of tetraploid lineages from the same two diploid progenitors are common, but here we report the first known instance of a single tetraploid species that originated
R Symula et al.
Molecular phylogenetics and evolution, 47(2), 569-580 (2008-03-20)
Geographic patterns of species diversity in southeast Australia have been attributed to changes in Pleistocene climate, but related phylogeographic patterns and processes are relatively understudied. 12S and 16S mitochondrial DNA sequences in Crinia signifera populations were used to infer historical
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique