S0266

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

• Synonyme(s) : 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane
• Formule linéaire : NH₂(CH₂)₃NH(CH₂)₄NH₂
• Numéro CAS: 124-20-9
• Poids moléculaire : 145.25
• Numéro Beilstein : 1698591
• Numéro CE : 204-689-0
• Numéro MDL: MFCD00008229
• Code UNSPSC : 12352200
• ID de substance PubChem : 24899434
• Nomenclature NACRES : NA.52

Propriétés

• Qualité: for molecular biology
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Pureté: ≥98%
• Forme: solid or liquid
• Poids mol.: 145.25 (AMU)
• Technique(s): cell culture | mammalian: suitable
• Indice de réfraction: n20/D 1.479 (lit.)
• Densité: 0.925 g/mL at 25 °C (lit.)
• Activité étrangère: DNase, RNase, and protease, none detected
• Température de stockage: room temp
• Chaîne SMILES: NCCCCNCCCN
• InChI: 1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
• Clé InChI: ATHGHQPFGPMSJY-UHFFFAOYSA-N
• Informations sur le gène: rat ... Ppm1a(24666)

Description

Description générale

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Application

Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.

Actions biochimiques/physiologiques

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T₄ polynucleotide kinase activity.

Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.

Attention

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Danger
• Mentions de danger: H314
• Conseils de prudence: P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405
• Classification des risques: Eye Dam. 1 - Skin Corr. 1B
• Code de la classe de stockage: 8A - Combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 233.6 °F - closed cup
• Point d'éclair (°C): 112 °C - closed cup
• Équipement de protection individuelle: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter