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Key Documents

RAB0223

Sigma-Aldrich

Human IFN γ ELISA Kit

for cell and tissue lysates

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.77

Espèces réactives

human

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 15 pg/mL
standard curve range: 20.6-15000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... IFNG(3458)

Catégories apparentées

Description générale

The Human IFN-γ ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human IFN-γ cell lysate and tissue lysate.

Immunogène

Recombinant Human IFN-γ

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human IFNγ ELISA Kit has been used in ELISA assay.

Actions biochimiques/physiologiques

IFNG (interferon-γ) is a type-2 interferon, which is produced by Th (T helper cells)-1 lymphocytes, CD8 cytotoxic lymphocytes, natural killer (NK) cells, and natural killer T (NKT) cells. It plays an important role in innate and adaptive immune responses, and helps in defense against bacterial infections, dimorphic yeast and some viral infections. High expression of IFNG is common in many autoimmune diseases, such as systemic lupus erythematosis, Sjögren syndrome, inflammatory bowel disease and multiple sclerosis.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Réf. du produit
Description
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  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)FDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)FDS
  • RABLYSIS12X Cell Lysis Buffer (Item J)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Association between B7-H1 and cervical cancer: B7-H1 impairs the immune response in human cervical cancer cells.
Tao J
Experimental and Therapeutic Medicine, 14(5), 4125-4133 (2017)
Yong-Chen Lu et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 20(13), 3401-3410 (2014-07-06)
Cancer immunotherapy with adoptive transfer of tumor-infiltrating lymphocytes (TIL) represents an effective treatment for patients with metastatic melanoma, with the objective regressions in up to 72% of patients in three clinical trials. However, the antigen targets recognized by these effective
Samuel T Haile et al.
Cancer immunology research, 2(7), 610-615 (2014-05-14)
Tumor cells use various methods of immunosuppression to overcome antitumor immunity. One such method is that of programmed death ligand-1 (PD-L1 or B7-H1), which upon binding its receptor PD-1 on T cells triggers apoptotic death of the activated T cells.
Vibha Jha et al.
PloS one, 9(8), e104484-e104484 (2014-08-15)
Environmental factors including drugs, mineral oils and heavy metals such as lead, gold and mercury are triggers of autoimmune diseases in animal models or even in occupationally exposed humans. After exposure to subtoxic levels of mercury (Hg), genetically susceptible strains
Shigeo Koido et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 20(16), 4228-4239 (2014-07-25)
We performed a phase I trial to investigate the safety, clinical responses, and Wilms' tumor 1 (WT1)-specific immune responses following treatment with dendritic cells (DC) pulsed with a mixture of three types of WT1 peptides, including both MHC class I
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