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P8124

Sigma-Aldrich

Pyoverdines

from Pseudomonas fluorescens, >90% (HPLC)

Synonyme(s) :

Pyoverdine Detection, Pyoverdine Protein, Siderophore Protein

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32

Source biologique

Pseudomonas fluorescens

Niveau de qualité

200

Pureté

>90% (HPLC)

Forme

powder

Solubilité

H2O: ~10 mg/mL

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Application

Pyoverdines from Pseudomonas fluorescens has been used :
  • in tryptophan fluorescence quenching to determine its binding to neutrophil-gelatinase-associated lipocalin (NGAL)
  • to detect pyoverdine diffusion surrounding siderophore-conjugated monobactam (MB-1)-resistant colonies in chelexed, dialyzed Mueller-Hinton broth (CDMHB)
  • for synchronous fluorescence spectra of purified pyoverdine

Actions biochimiques/physiologiques

Pyoverdines help in promoting plant growth by chelating iron and rendering it unavailable for plant pathogens. The ferri-pyoverdine complex acts as a signaling molecule inducing the production of secreted virulence factors in Pseudomonas aeruginosa.
Pyoverdines, also called pseuobactins and pyoverdins, are fluorescent siderophores that have high affinity for iron (1032 M-1), and are synthesized by fluorescent pseudomonads under iron-deficient growth conditions. Pyoverdines were shown to prevent iron toxicity produced by iron overload in hepatocyte cultures and effectively scavenges the hydroxyl and peroxyl radicals. Pyoverdines are effective in acquiring iron from transferrin and lactoferrin. Pyoverdines are also involved in the suppression of pythium-induced damping-off of tomato and promotion of growth in some higher plants.

Forme physique

Supplied as a mixture containing mainly the succinic acid (MW=1161), 2-hydroxy glutaramide (MW=1190), and succinamide (MW=1160) forms of pyoverdines.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Adaptation-based resistance to siderophore-conjugated antibacterial agents by Pseudomonas aeruginosa.
Tomaras AP
Antimicrobial Agents and Chemotherapy, 57(9), 4197-4207 (2013)
Mylene Vaillancourt et al.
PLoS pathogens, 19(4), e1010942-e1010942 (2023-04-08)
During chronic cystic fibrosis (CF) infections, evolved Pseudomonas aeruginosa antibiotic resistance is linked to increased pulmonary exacerbations, decreased lung function, and hospitalizations. However, the virulence mechanisms underlying worse outcomes caused by antibiotic resistant infections are poorly understood. Here, we investigated
Correction: Multicolor Whole-Cell Bacterial Sensing Using a Synchronous Fluorescence Spectroscopy-Based Approach.
PLoS ONE (2015)
Pyoverdine, the Major Siderophore in Pseudomonas aeruginosa, Evades NGAL Recognition.
Peek ME
Interdisciplinary perspectives on infectious diseases, 843509-843509 (2012)
Purification of Pyoverdines of Pseudomonas fluorescens 2-79 by Copper-Chelate Chromatography.
Xiao R and Kisaalita WS
Applied and Environmental Microbiology, 61(11), 3769-3774 (1995)
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