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P6675

Sigma-Aldrich

Prolidase from porcine kidney

lyophilized powder, ≥100 units/mg protein

Synonyme(s) :

Aminoacyl-L-proline hydrolase, Imido Dipeptidase, Prolidase, Proline dipeptidase

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About This Item

Numéro CAS:
9025-32-5
Numéro de classification (Commission des enzymes):
3.4.13.9 (BRENDA, IUBMB)
Numéro CE :
232-791-5
Numéro MDL:
MFCD00132079
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Forme

lyophilized powder

Niveau de qualité

200

Activité spécifique

≥100 units/mg protein

Composition

Protein, 20-74% Lowry

Température de stockage

−20°C

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Catégories apparentées

Description générale

Prolidase is a cytosolic exopeptidase. It is a homodimeric enzyme which requires divalent cations like manganese as a cofactor in its active site for its function.

Application

Prolidase from porcine kidney has been used:
  • in the enzymatic hydrolysis of porcine milk for the recovery of L-glutamine from proteins and peptides
  • in the proteolysis of skim milk for the determination of ε-(γ-glutamyl)lysine and free aminoacids
  • to determine its effect on the activity of enterococcin A 2000

Prolidase has an important role in recycling of proline and collagen production. It is used to study mutations in the PEPD gene that cause prolidase deficiency. It is used to hydrolyze proteins with C-terminal proline or hydroxyproline residues. Prolidase, product P6675 from porcine kidney, has been used to hydrolyze peptide bonds from the amino terminus when studying enzymatic methylation of membrane proteins.

Actions biochimiques/physiologiques

Prolidase is an enzyme that catalyzes the hydrolysis of the imide bond between an α-carboxyl group and proline or hydroxyproline. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues.
Rare mutation in prolidase gene causes deficiency leading to massive imidodipeptiduria, elevated proline-containing dipeptides in plasma, recurrent infections, mental retardation and skin lesions.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of Gly-Pro per min at pH 8.0 at 40 °C.

Forme physique

Supplied as a lyophilized powder containing Tris buffer salt and MnCl2.

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

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L-Glutamine or L-alanyl-L-glutamine prevents oxidant-or endotoxin-induced death of neonatal enterocytes
Haynes TE, et al.
Amino Acids, 37(1), 131-142 (2009)
Ertugrul Uzar et al.
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, 33(4), 875-880 (2011-11-29)
We found no data in the literature related to oxidative stress index (OSI), total oxidative status (TOS) and prolidase activity in patients with diabetic neuropathy (DN). In this study, we aimed to evaluate the oxidative status of DN patients via
Prolidase
Namiduru, ES
Bratislavske lekarske Listy, 117(8), 480-485 (2016)
Mehmet A Altay et al.
Scandinavian journal of clinical and laboratory investigation, 71(7), 576-582 (2011-08-13)
We aimed to investigate serum prolidase activity and to find out its association with oxidative-antioxidative status in patients with idiopathic clubfoot and during the course of the disease. Oxidative status parameters, including total free sulfhydryl groups (-SH), total antioxidant capacity
Isolation and partial characterization of an antibacterial substance produced by Enterococcus faecium
Pantev A, et al.
Folia Microbiologica, 47(4), 391-400 (2002)
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