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P6097

Sigma-Aldrich

Precast Agarose Gels

24 wells (2 × 12-well portrait orientation)

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About This Item

Code UNSPSC :
41105300
Nomenclature NACRES :
NB.22

Qualité

for molecular biology

Niveau de qualité

100

Forme

gel form

Composition

Température de stockage

room temp

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Description générale

Precast gels contain 4.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide. RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0) Quality

Application

Precast agarose gels are designed for high resolution separation of nucleic acids, producing sharp DNA and RNA bands with low background fluorescence. Precast gels are individually foil sealed in plastic trays that help eliminate contamination from equipment and handling. Gels can be electrophoresed right in the plastic tray.

TBE - agarose gels are available in two common concentrations (1% and 4%) for DNA electrophoresis. Ethidium bromide (0.5 μg/ml) is included in the TBE-agarose gels for easy visualization. TBE-agarose gels are available in 8, 20 and 24 well formats. The separation range for the 1.0% gel is 500-1000 bp and 8-1000 bp for the 4% gel.

MOPS - agarose gels (1.25%) are available without denaturants (e.g. formaldehyde or glyoxal) for the analysis of total RNA, in vitro RNA transcripts, and Northern blotting. RNA electrophoresis usually does not interfere with staining. If significant secondary structure is suspected, the RNA should be denatured by a method listed in the product insert. The separation range for the 1.25% MOPS gel is 0.25-10 kb.
Suitable for separation of DNA fragments ranging from 8 – 1000 base pairs.

Conditionnement

Package of 20 individually foil-wrapped gels.

Composants

Precast gels contain 4.0% agarose in 1X TBE buffer with 0.5 μg/ml ethidium bromide.

Autres remarques

DNA agarose gels contain 1× TBE buffer (0.089 M Tris-borate, pH 8.3, 2 mM EDTA) and 0.5 mg/ml ethidium bromide.
RNA agarose gels contain 1× MOPS (20 mM MOPS, 5 mM sodium acetate, 1 mM EDTA and 1 mM EGTA, pH 7.0)

Qualité

No detected DNase or RNase activity

Forme physique

Gel size: 6.0 × 9.5 cm, 5.5 mm thick
Cassette size: 6.8 × 10.2 cm container tray; fits common horizontal electrophoresis units
Sample format: 8-well (portrait), 20-well (landscape), 24-well (2 × 12 well portrait)

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

D9156

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D3160

Deoxyribonucleic acid from human placenta, buffered aqueous solution, sexed, male

S8140

SSPE Buffer, for Northern and Southern blotting, powder blend

P5722

Precast Agarose Gels, 20-well (landscape orientation)

N6019

Neutralizing Buffer, for Northern and Southern blotting, powder

N1531

Denaturation Solution, for Northern and Southern blotting

G7654

Gel Loading Solution, for NA electrophoresis, solution

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

S2015

SSPE Buffer 20× Concentrate, for Northern and Southern blotting, solution

G2526

Gel Loading Buffer, for NA electrophoresis

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

S0902

SSC Buffer, for Northern and Southern blotting, powder blend

H7033

Tampon d′hybridation PerfectHyb™ Plus, for Northern and Southern blotting, solution

S8015

SSC Buffer, for Northern and Southern blotting, powder blend

P5972

Precast Agarose Gels, 24 wells (2 × 12-well portrait orientation)

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

P5472

Precast Agarose Gels, 8-well (portrait orientation)

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

C7594

Casein Blocking Buffer, for Northern and Southern blotting, powder blend

15356

Blotting-Nylon 66 membranes, type B, positive, for Northern and Southern blotting

31149

Deoxyribonucleic acid, low molecular weight from salmon sperm

18470

Bromothymol Blue sodium salt, for microscopy (Bot., Hist., Vit.), indicator (pH 6.0-7.6)

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Precast Agarose Gels

These gels are suitable for separating nucleic acids, giving sharp DNA bands and low background fluorescence.

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Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

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